{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:15:30Z","timestamp":1790248530309,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2005,2,1]],"date-time":"2005-02-01T00:00:00Z","timestamp":1107216000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The etiological agent most commonly associated with bacillary dysentery is\n            <jats:italic>Shigella<\/jats:italic>\n            . As part of its mandate, the Bacteriology and Enteric Disease Program of Health Canada identifies and serotypes unusual isolates of\n            <jats:italic>Shigella<\/jats:italic>\n            received from provincial laboratories of public health. In this report, six unusual isolates from three provinces were analyzed biochemically and serologically using slide and tube agglutinations and molecularly using standard pulsed-filed gel electrophoresis (PFGE), PCR, and PCR-restriction fragment length polymorphism (RFLP) techniques. All six isolates were identical. PFGE analysis grouped these strains; biochemically, they were mannitol negative and consistent with the profile of\n            <jats:italic>Shigella<\/jats:italic>\n            . Serologically, these strains produced weak reactions in\n            <jats:italic>Shigella dysenteriae<\/jats:italic>\n            serovars 4 and 16 and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            O159 and O173 antisera. Molecular serotyping by PCR-RFLP of the\n            <jats:italic>rfb<\/jats:italic>\n            gene produced an\n            <jats:italic>S. dysenteriae<\/jats:italic>\n            serovar 2\/\n            <jats:italic>E. coli<\/jats:italic>\n            O112ac pattern. They were positive by PCR for\n            <jats:italic>ipaH<\/jats:italic>\n            and\n            <jats:italic>ial<\/jats:italic>\n            enteroinvasive genes but negative for all other genes tested. Antiserum was prepared from one of the isolates and tested against\n            <jats:italic>Shigella<\/jats:italic>\n            and\n            <jats:italic>E. coli<\/jats:italic>\n            reference strains as well as the other isolates. The antiserum reacted with the five remaining isolates and showed cross-reactivity with\n            <jats:italic>S. dysenteriae<\/jats:italic>\n            serovars 1, 4, and 16;\n            <jats:italic>Shigella flexneri<\/jats:italic>\n            type 3; and\n            <jats:italic>E. coli<\/jats:italic>\n            O118, O159, O168, O172, and O173 antigens. Absorbing the sera with\n            <jats:italic>E. coli<\/jats:italic>\n            O159 and\n            <jats:italic>S. dysenteriae<\/jats:italic>\n            serovar 4 antigen removed all cross-reactions and only slightly reduced the homologous titer. Based on biochemical, molecular, and complete serological analysis, we propose that these six isolates represent a new provisional serovar of\n            <jats:italic>S. dysenteriae<\/jats:italic>\n            , type strain BEDP 02-5104.\n          <\/jats:p>","DOI":"10.1128\/jcm.43.2.740-744.2005","type":"journal-article","created":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T21:42:56Z","timestamp":1107553376000},"page":"740-744","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["A Novel\n            <i>Shigella dysenteriae<\/i>\n            Serovar Isolated in Canada"],"prefix":"10.1128","volume":"43","author":[{"given":"P. L.","family":"Melito","sequence":"first","affiliation":[{"name":"Bacteriology and Enteric Disease Program, National Microbiology Laboratory, Winnipeg, Manitoba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. L.","family":"Woodward","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Disease Program, National Microbiology Laboratory, Winnipeg, Manitoba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Munro","sequence":"additional","affiliation":[{"name":"Health Canada, Pest Management Regulatory Agency, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Disease Program, National Microbiology Laboratory, Winnipeg, Manitoba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Foster","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Disease Program, National Microbiology Laboratory, Winnipeg, Manitoba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Tilley","sequence":"additional","affiliation":[{"name":"Provincial Laboratory of Public Health for Southern Alberta, Calgary, Alberta"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Paccagnella","sequence":"additional","affiliation":[{"name":"British Columbia Centre for Disease Control, Vancouver, British Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Isaac-Renton","sequence":"additional","affiliation":[{"name":"British Columbia Centre for Disease Control, Vancouver, British Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ismail","sequence":"additional","affiliation":[{"name":"Laboratoire de Sante\u0301 Publique du Que\u0301bec, Institutes de Nationale Sante\u0301 Publique du Que\u0301bec, Que\u0301bec"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. K.","family":"Ng","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Disease Program, National Microbiology Laboratory, Winnipeg, Manitoba"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Allen J. K. C. G. Clark D. L. Woodward R. A. Foster H. A. Tabor and F. G. Rodgers. 2002. Extension of the rfb -RFLP method for determining O-serogroups of Escherichia coli and Shigella spp. poster presentation C-268. 102nd Gen. Meet. Am Soc. Microbiol. 2002. American Society for Microbiology Washington D.C."},{"key":"e_1_3_2_3_2","first-page":"654","year":"2003","unstructured":"Bopp, C. A., F. W. Brenner, P. I. Fields, J. G. Wells, and N. A. Strockbine. 2003. Escherichia, Shigella, and Salmonella, p. 654-671. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller, and R. H. Yolken (ed.), Manual of clinical microbiology, 8th ed. 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