{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T00:14:03Z","timestamp":1715991243116},"reference-count":24,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2005,2,1]],"date-time":"2005-02-01T00:00:00Z","timestamp":1107216000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Since mid-1991, New Zealand has experienced an epidemic of meningococcal disease. The epidemic has been caused by serogroup B meningococci expressing PorA type P1.7-2,4, belonging to the ST-41\/ST-44 complex, lineage III. Most B:P1.7-2,4 meningococci express type 4 PorB (87.0%), although case isolates with\n porB<\/jats:italic>\n other than type 4 have been identified throughout the duration of the epidemic. To assess the genetic relatedness of case isolates with an alternative\n porB<\/jats:italic>\n gene, multilocus restriction typing validated against multilocus sequence typing was used. This determined that B:P1.7-2,4 meningococci with a\n porB<\/jats:italic>\n gene that was other than type 4 had the same clonal origin. It was concluded that strains with alternative\n porB<\/jats:italic>\n genes had diverged from the original type 4\n porB<\/jats:italic>\n . Variation in\n porB<\/jats:italic>\n was also shown to be associated with the uptake of DNA encoding one or two of the PorB variable regions leading to mosaic\n porB<\/jats:italic>\n . Point mutation rather than horizontal transfer and recombination was implicated as the mechanism of sequence variation in some strains. This work will serve as a reference point to determine if the administration of a strain-specific vaccine increases the level of\n porB<\/jats:italic>\n divergence and variation already observed in New Zealand case isolates. It also complements the study undertaken of PorA stability which showed that variation in P1.7-2,4 PorA was almost exclusively due to deletions in the P1.4 epitope of the epidemic strain.\n <\/jats:p>","DOI":"10.1128\/jcm.43.2.838-842.2005","type":"journal-article","created":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T21:42:56Z","timestamp":1107553376000},"page":"838-842","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Sequence Variation in the\n porB<\/i>\n Gene from B:P1.4 Meningococci Causing New Zealand's Epidemic"],"prefix":"10.1128","volume":"43","author":[{"given":"Kristin H.","family":"Dyet","sequence":"first","affiliation":[{"name":"Communicable Disease Group, Institute of Environmental Science and Research, Porirua, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana R.","family":"Martin","sequence":"additional","affiliation":[{"name":"Communicable Disease Group, Institute of Environmental Science and Research, Porirua, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(88)90045-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.3842-3846.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.4.1484-1490.1995"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01519.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05225-0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1998.tb01379.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.2.832-837.2005"},{"key":"e_1_3_2_9_2","unstructured":"Dyet K. H. 2003. Measurement of genetic heterogeneity in serogroup B meningococci in the context of New Zealand's epidemic. Ph.D. thesis. University of Otago Dunedin New Zealand."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1731-1733.2004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1742-1745.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.9.3620-3629.1992"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/7.4.504"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","unstructured":"Holst J. I. S. Aaberge P. Oster D. Lennon D. Martin J. O'Hallahan K. Nord H. N\u00f8kelby L. M. N\u00e6ss K. M\u00f8yner P. Kristiansen A. G. Skryten K. Byrn A. Aase R. Rappuoli and E. Rosenqvist. 24 July 2003 posting date. A \u2018tailor-made\u2019 vaccine trialled as part of public health response to group B meningococcal disease epidemic in New Zealand. Eurosurveillance Wkly. 7 : 5. 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