{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:47:14Z","timestamp":1790423234885,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Escherichia coli<\/jats:italic>\n            strain TUM2139 was isolated from a stool sample from a 9-year-old girl on 16 June 2004. This strain was categorized as Shiga toxin-producing\n            <jats:italic>Escherichia coli<\/jats:italic>\n            (STEC) because the Shiga-like toxin gene\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>1<\/jats:sub>\n            was detected by immunochromatography and PCR assay. The strain was highly resistant to cefotaxime (256 \u03bcg\/ml) and was also resistant to cefepime, cefpodoxime, ceftriaxone, and aztreonam. In the presence of 4 \u03bcg of clavulanic acid per ml, the MIC of cefotaxime decreased to \u22640.12 \u03bcg\/ml, indicating that this strain was an extended-spectrum \u03b2-lactamase (ESBL) producer. Cefotaxime resistance was transferred to\n            <jats:italic>E. coli<\/jats:italic>\n            C600 by conjugation at a frequency of 3.0 \u00d7 10\n            <jats:sup>\u22126<\/jats:sup>\n            . A PCR assay was performed with primer sets specific for TEM-type and SHV-type ESBLs and for the CTX-M-2 (Toho-1), CTX-M-3, and CTX-M-9 groups of ESBLs. A specific signal was observed with the primer set specific for the CTX-M-9 group of \u03b2-lactamases. This \u03b2-lactamase was confirmed to be the ESBL CTX-M-18 by DNA sequencing. This is the first report of an ESBL-producing STEC isolate.\n          <\/jats:p>","DOI":"10.1128\/jcm.43.3.1072-1075.2005","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T23:47:54Z","timestamp":1109980074000},"page":"1072-1075","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Extended-Spectrum \u03b2-Lactamase-Producing Shiga Toxin Gene (\n            <i>stx<\/i>\n            <sub>1<\/sub>\n            )-Positive\n            <i>Escherichia coli<\/i>\n            O26:H11: a New Concern"],"prefix":"10.1128","volume":"43","author":[{"given":"Yoshikazu","family":"Ishii","sequence":"first","affiliation":[{"name":"Department of Microbiology, Toho University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soichiro","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Toho University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimena","family":"Alba","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Toho University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuaki","family":"Shiroto","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Toho University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanobu","family":"Otsuka","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Toho University Ohashi Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naotaka","family":"Hashizume","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Toho University Ohashi Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumichi","family":"Tamura","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keizo","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Toho University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.1.169"},{"key":"e_1_3_2_3_2","first-page":"321","volume":"289","year":"1980","unstructured":"Ambler, R. 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