{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T17:54:58Z","timestamp":1783792498243,"version":"3.55.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T00:00:00Z","timestamp":1112313600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>We report the development of nucleic acid sequence-based amplification (NASBA) and quantitative real-time reverse transcription (RT)-PCR assays for the detection of La Crosse (LAC) virus in field-collected vector mosquito samples and human clinical samples. The sensitivities of these assays were compared to that of a standard plaque assay in Vero cells. The NASBA and quantitative real-time RT-PCR assays demonstrated sensitivities greater than that of the standard plaque assay. The specificities of these assays were determined by testing a battery of reference strain viruses, including representative strains of LAC virus and other arthropod-borne viruses. Additionally, these assays were used to detect LAC viral RNA in mosquito pool samples and human brain tissue samples and yielded results within less than 4 h. The NASBA and quantitative real-time RT-PCR assays detect LAC viral RNA in a sensitive, specific, and rapid manner; these findings support the use of these assays in surveillance and diagnostic laboratory systems.<\/jats:p>","DOI":"10.1128\/jcm.43.4.1885-1889.2005","type":"journal-article","created":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T23:32:50Z","timestamp":1112830370000},"page":"1885-1889","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Nucleic Acid Amplification Assays for Detection of La Crosse Virus RNA"],"prefix":"10.1128","volume":"43","author":[{"given":"Amy J.","family":"Lambert","sequence":"first","affiliation":[{"name":"Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger S.","family":"Nasci","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce C.","family":"Cropp","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise A.","family":"Martin","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Becky C.","family":"Rose","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandy J.","family":"Russell","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S.","family":"Lanciotti","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"27","volume":"169","year":"1991","unstructured":"Beaty, B. 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