{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T15:28:54Z","timestamp":1762874934742},"reference-count":22,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T00:00:00Z","timestamp":1112313600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.\n          <\/jats:p>","DOI":"10.1128\/jcm.43.4.2004-2008.2005","type":"journal-article","created":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T23:32:50Z","timestamp":1112830370000},"page":"2004-2008","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Multilocus Sequence Typing Directly on DNA from Clinical Samples and a Cultured Isolate To Investigate Linked Fatal Pneumococcal Disease in Residents of a Shelter for Homeless Men"],"prefix":"10.1128","volume":"43","author":[{"given":"Andrew","family":"Birtles","sequence":"first","affiliation":[{"name":"Health Protection Agency, Respiratory and Systemic Infections Laboratory, Central Public Health Laboratory, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noel","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Health Protection Agency, South East Region"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen L.","family":"Sheppard","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Respiratory and Systemic Infections Laboratory, Central Public Health Laboratory, London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry","family":"Rutter","sequence":"additional","affiliation":[{"name":"South East Public Health Observatory"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm","family":"Guiver","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Meningococcal Reference Unit, North West Regional Laboratory, Manchester, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Haworth","sequence":"additional","affiliation":[{"name":"Health Protection Agency, South East Region"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C.","family":"George","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Respiratory and Systemic Infections Laboratory, Central Public Health Laboratory, London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"24","volume":"28","year":"2002","unstructured":"Azadan, R. J., J. C. Fogleman, and P. B. Danielson. 2002. Capillary electrophoresis sequencing: maximum read length at minimal cost. BioTechniques28:24-26.","journal-title":"BioTechniques"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2378-2384.2003"},{"key":"e_1_3_2_4_2","first-page":"133","volume":"7","year":"1995","unstructured":"Burman, L. A., R. Norrby, and B. Trollfors. 1995. Invasive pneumococcal infections: incidence, predisposing factors, and prognosis. Rev. Infect. Dis.7:133-142.","journal-title":"Rev. Infect. Dis."},{"key":"e_1_3_2_5_2","first-page":"1","volume":"46","year":"1997","unstructured":"Centers for Disease Control and Prevention (CDC). 1997. Prevention of pneumococcal disease. Recommendations of the Advisory Committee on Immunization Practices (ACIP). Morb. Mortal. Wkly. Rep.46(RR-08):1-24.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1553-1558.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1980.03310130024022"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-11-3049"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960000321"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1997.tb04500.x"},{"key":"e_1_3_2_11_2","first-page":"498","volume":"20","year":"2000","unstructured":"Hoskins, J., W. E. Alborn, Jr., J. Arnold, L. C. Blaszczak, S. Burgett, B. S. DeHoff, S. T. Estrem, L. Fritz, D. J. Fu, W. Fuller, C. Geringer, R. Gilmour, J. S. Glass, H. Khoja, A. R. Kraft, R. E. Lagace, D. J. LeBlanc, L. N. Lee, E. J. Lefkowitz, J. Lu, P. Matsushima, S. M. McAhren, M. McHenney, K. McLeaster, C. W. Mundy, T. I. Nicas, F. H. Norris, M. O'Gara, R. B. Peery, G. T. Robertson, P. Rockey, P. M. Sun, M. E. Winkler, Y. Yang, M. Young-Bellido, G. Zhao, C. A. Zook, R. H. Baltz, S. R. Jaskunas, P. R. Rosteck, Jr., P. L. Skatrud, and J. I. Glass. 2000. Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science20:498-506.","journal-title":"Science"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05277-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.601-607.2003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.3140"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022521"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-49-4-361"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/517764"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.99.1.147"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200003093421002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/514589"},{"key":"e_1_3_2_21_2","unstructured":"Salisbury D. M. and N. T. Begg (ed.). 1996. Immunisation against infectious disease. Her Majesty's Stationery Office London United Kingdom."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2003.08.006"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/322676"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.43.4.2004-2008.2005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.43.4.2004-2008.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T04:05:52Z","timestamp":1646453152000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.43.4.2004-2008.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2005,4]]}},"alternative-id":["10.1128\/JCM.43.4.2004-2008.2005"],"URL":"https:\/\/doi.org\/10.1128\/jcm.43.4.2004-2008.2005","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4]]},"assertion":[{"value":"2004-07-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-12-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}