{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:41:25Z","timestamp":1759333285199},"reference-count":25,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2005,8,1]],"date-time":"2005-08-01T00:00:00Z","timestamp":1122854400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The survival and microevolution of\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            strains in the niches of the stomach after eradication therapy have largely been unexplored. We analyzed genomic signatures for two successive isolates obtained 9 years apart from a duodenal ulcer patient who underwent eradication therapy for\n            <jats:italic>H. pylori<\/jats:italic>\n            . These isolates were genotyped based on 50 different parameters involving three different fingerprinting approaches and several evolutionarily significant and virulence-associated landmarks in the genome, including nine informative gene loci, the\n            <jats:italic>cag<\/jats:italic>\n            pathogenicity island and its right junction, members of the plasticity region cluster, and\n            <jats:italic>vacA<\/jats:italic>\n            and\n            <jats:italic>iceA<\/jats:italic>\n            alleles. Our observations reveal that the two isolates were derived from the same strain that colonized the patient for almost a decade and were almost identical. Microevolution, however, was observed in the\n            <jats:italic>cagA<\/jats:italic>\n            gene and its right junction, the\n            <jats:italic>vacA<\/jats:italic>\n            m1 allele, and a member of the plasticity region cluster (JHP926). These results suggest that\n            <jats:italic>H. pylori<\/jats:italic>\n            has a great ability to survive and reemerge as a microevolved strain posteradication, thereby hinting at the requirement for follow-up of patients after therapy.\n          <\/jats:p>","DOI":"10.1128\/jcm.43.8.4237-4241.2005","type":"journal-article","created":{"date-parts":[[2005,8,4]],"date-time":"2005-08-04T22:25:57Z","timestamp":1123194357000},"page":"4237-4241","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Pathogen Evolution In Vivo: Genome Dynamics of Two Isolates Obtained 9 Years Apart from a Duodenal Ulcer Patient Infected with a Single\n            <i>Helicobacter pylori<\/i>\n            Strain"],"prefix":"10.1128","volume":"43","author":[{"given":"Vale\u0301rie","family":"Prouzet-Maule\u0301on","sequence":"first","affiliation":[{"name":"INSERM ERI 10 and Centre National de Re\u0301fe\u0301rence des Campylobacters et He\u0301licobacters, Laboratoire de Bacte\u0301riologie, Universite\u0301 Victor Segalen Bordeaux 2, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Abid","family":"Hussain","sequence":"additional","affiliation":[{"name":"Pathogen Evolution Group, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herve\u0301","family":"Lamouliatte","sequence":"additional","affiliation":[{"name":"Service He\u0301pato-Gastroente\u0301rologie, Ho\u0302pital Saint Andre\u0301, Bordeaux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhana","family":"Kauser","sequence":"additional","affiliation":[{"name":"Pathogen Evolution Group, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis","family":"Me\u0301graud","sequence":"additional","affiliation":[{"name":"INSERM ERI 10 and Centre National de Re\u0301fe\u0301rence des Campylobacters et He\u0301licobacters, Laboratoire de Bacte\u0301riologie, Universite\u0301 Victor Segalen Bordeaux 2, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niyaz","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Pathogen Evolution Group, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2003.07424.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.1.116-121.1995"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.19.5137"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.9.2979-2982.1999"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.15.5182-5185.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241517298"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05263-0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.720"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251396098"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.242574599"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-005-1613-1"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.6.2372-2378.2004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.1.181-186.2001"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251551698"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5302-5308.2004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01140.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.11.3210-3218.2000"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/315173"},{"key":"e_1_3_2_20_2","first-page":"1311","volume":"16","year":"1984","unstructured":"Marshall, B. 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