{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:03:03Z","timestamp":1785369783898,"version":"3.55.0"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2005,9,1]],"date-time":"2005-09-01T00:00:00Z","timestamp":1125532800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            During the anthrax attack of 2001, the Florida Department of Health (FDOH) Bureau of Laboratories in Tampa received hundreds of isolates suspected of being\n            <jats:italic>Bacillus anthraci<\/jats:italic>\n            s. None were confirmed to be\n            <jats:italic>B. anthracis<\/jats:italic>\n            since most isolates were motile and not even in the\n            <jats:italic>Bacillus cereus<\/jats:italic>\n            group. Although the sentinel laboratories now send fewer isolates to FDOH laboratories, should another attack occur the number of isolates submitted would likely increase dramatically, and this upsurge would seriously challenge personnel who are expected to be busy examining an increased number of environmental samples. We examined two selective and differential growth media and alternative motility methods that could be used to streamline the processing of suspicious isolates. Of 60 isolates previously sent to the FDOH laboratory, 56 were endospore-forming gram-positive rods and only 7 grew on mannitol-egg yolk-polymyxin B agar and\/or the\n            <jats:italic>Anthracis<\/jats:italic>\n            chromogenic agar. Microscopic observation of early-log-phase growth (2 to 3 h) in a shaking broth was the best method to detect motility in 40 isolates that appeared nonmotile in the motility media investigated. One of these growth media and microscopic examination of shaken broth cultures can be used to show that an isolate is not\n            <jats:italic>B. anthracis<\/jats:italic>\n            before expensive molecular and antibody-based tests are performed. By doing so, costs could be reduced and analysis time shortened.\n          <\/jats:p>","DOI":"10.1128\/jcm.43.9.4336-4341.2005","type":"journal-article","created":{"date-parts":[[2005,9,6]],"date-time":"2005-09-06T21:58:02Z","timestamp":1126043882000},"page":"4336-4341","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Use of Two Selective Media and a Broth Motility Test Can Aid in Identification or Exclusion of\n            <i>Bacillus anthracis<\/i>"],"prefix":"10.1128","volume":"43","author":[{"given":"Vicki A.","family":"Luna","sequence":"first","affiliation":[{"name":"Center for Biological Defense, College of Public Health, University of South Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Kealy","family":"Peak","sequence":"additional","affiliation":[{"name":"Center for Biological Defense, College of Public Health, University of South Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William O.","family":"Veguilla","sequence":"additional","affiliation":[{"name":"Center for Biological Defense, College of Public Health, University of South Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Reeves","sequence":"additional","affiliation":[{"name":"Florida Department of Health, Bureau of Laboratories, Tampa, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Heberlein-Larson","sequence":"additional","affiliation":[{"name":"Florida Department of Health, Bureau of Laboratories, Tampa, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew C.","family":"Cannons","sequence":"additional","affiliation":[{"name":"Center for Biological Defense, College of Public Health, University of South Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phil","family":"Amuso","sequence":"additional","affiliation":[{"name":"Center for Biological Defense, College of Public Health, University of South Florida"},{"name":"Florida Department of Health, Bureau of Laboratories, Tampa, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Cattani","sequence":"additional","affiliation":[{"name":"Center for Biological Defense, College of Public Health, University of South Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"665","year":"1993","unstructured":"Atlas, R. M. 1993. NSMP, modified, p. 665-666. In L. C. Parks (ed.), Handbook of microbiological media. CRC Press, Inc., Boca Raton, Fla.","journal-title":"Handbook of microbiological media"},{"key":"e_1_3_2_3_2","first-page":"889","volume":"50","year":"2001","unstructured":"Centers for Disease Control and Prevention. 2001. Update: investigation of anthrax associated with intentional exposure and interim public health guidelines, October 2001. Morbid. Mortal. Wkly. Rep.50:889-893.","journal-title":"Morbid. Mortal. Wkly. Rep."},{"key":"e_1_3_2_4_2","first-page":"909","volume":"50","year":"2001","unstructured":"Centers for Disease Control and Prevention. 2001. Investigation of bioterrorism-related anthrax and interim guidelines for exposure management and antimicrobial therapy, October 2001. Morb. Mortal. Wkly. Rep.50:909-919.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_5_2","first-page":"529","year":"1974","unstructured":"Gibson, T., and R. E. Gordon. 1974. Endospore-forming rods and cocci, p. 529-575. In R. E. Buchanan and N. E. Gibbons (ed.), Bergey's manual of determinative bacteriology, 8th ed. The Williams & Wilkins Company, Baltimore, Md.","journal-title":"Bergey's manual of determinative bacteriology"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63221-0"},{"key":"e_1_3_2_7_2","first-page":"357","year":"1999","unstructured":"Logan, N. A., and P. C. B. Turnbull. 1999. Bacillus and recently derived genera, p. 357-369. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.","journal-title":"Manual of clinical microbiology"},{"key":"e_1_3_2_8_2","first-page":"445","year":"2003","unstructured":"Logan, N. A., and P. C. B. Turnbull. 2003. Bacillus and other aerobic endospore-forming bacteria, p. 445-460. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller, and R. H. Yolken (ed.), Manual of clinical microbiology, 8th ed. American Society for Microbiology, Washington, D.C.","journal-title":"Manual of clinical microbiology"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0706.010604"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0810.020353"},{"key":"e_1_3_2_11_2","first-page":"273","year":"2000","unstructured":"MacFaddin, J. F. 2000. Lecithinase test, p. 273-281. In L. McGrew (ed.), Biochemical tests for identification of medical bacteria, 3rd ed. Lippincott\/The Williams & Wilkins, Philadelphia, Pa.","journal-title":"Biochemical tests for identification of medical bacteria"},{"key":"e_1_3_2_12_2","first-page":"384","volume":"4","year":"1962","unstructured":"Smirnoff, W. A. 1962. A staining method for differentiating spores, crystals, and cells of Bacillus thuringiensis (Berliner). J. Insect Pathol.4:384-386.","journal-title":"J. Insect Pathol."},{"key":"e_1_3_2_13_2","first-page":"1104","volume":"2","year":"1986","unstructured":"Sneath, P. H. A. 1986. Endospore-forming gram-positive rods and cocci, p. 1104-1139. In P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt (ed.), Bergey's manual of systematic bacteriology, vol. 2. The Williams & Wilkins Company, Baltimore, Md.","journal-title":"Bergey's manual of systematic bacteriology"},{"key":"e_1_3_2_14_2","first-page":"47","year":"2003","unstructured":"Weyant, R. S., J. W. Ezzell, and T. Popovic. 2003. Level B. Laboratory procedure for the identification of Bacillus anthracis, p. 47-64. In K. Q. Lindsey and S. A. Morse (ed.), Laboratory protocols for bioterrorism response laboratories for the identification of Bacillus anthracis. [Online.] www.lrnb.cdc.gov .","journal-title":"Laboratory protocols for bioterrorism response laboratories for the identification of Bacillus anthracis."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.43.9.4336-4341.2005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.43.9.4336-4341.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T04:19:30Z","timestamp":1646453970000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.43.9.4336-4341.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9]]},"references-count":13,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2005,9]]}},"alternative-id":["10.1128\/JCM.43.9.4336-4341.2005"],"URL":"https:\/\/doi.org\/10.1128\/jcm.43.9.4336-4341.2005","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9]]},"assertion":[{"value":"2005-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}