{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:05:36Z","timestamp":1791947136466,"version":"4.3.4"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Mucormycosis is an emerging infection associated with a high mortality rate. Identification of the causative agents remains difficult and time-consuming by standard mycological procedures. In this study, internal transcribed spacer (ITS) sequencing was validated as a reliable technique for identification of Zygomycetes to the species level. Furthermore, species identification directly from infected tissues was evaluated in experimentally infected mice. Fifty-four Zygomycetes strains belonging to 16 species, including the most common pathogenic species of\n            <jats:italic>Rhizopus<\/jats:italic>\n            spp.,\n            <jats:italic>Absidia<\/jats:italic>\n            spp.,\n            <jats:italic>Mucor<\/jats:italic>\n            spp., and\n            <jats:italic>Rhizomucor<\/jats:italic>\n            spp., were used to assess intra- and interspecies variability. Ribosomal DNA including the complete ITS1-5.8S-ITS2 region was amplified with fungal universal primers, sequenced, and compared. Overall, for a given species, sequence similarities between isolates were &gt;98%. In contrast, ITS sequences were very different between species, allowing an accurate identification of Zygomycetes to the species level in most cases. Six species (\n            <jats:italic>Rhizopus oryzae<\/jats:italic>\n            ,\n            <jats:italic>Rhizopus microsporus<\/jats:italic>\n            ,\n            <jats:italic>Rhizomucor pusillus<\/jats:italic>\n            ,\n            <jats:italic>Mucor circinelloides<\/jats:italic>\n            , and\n            <jats:italic>Mucor indicus<\/jats:italic>\n            ) were also used to induce disseminated mucormycosis in mice and to demonstrate that DNA extraction, amplification of fungal DNA, sequencing, and molecular identification were possible directly from frozen tissues.\n          <\/jats:p>","DOI":"10.1128\/jcm.44.2.340-349.2006","type":"journal-article","created":{"date-parts":[[2006,2,2]],"date-time":"2006-02-02T18:33:16Z","timestamp":1138905196000},"page":"340-349","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":158,"title":["Molecular Identification of Zygomycetes from Culture and Experimentally Infected Tissues"],"prefix":"10.1128","volume":"44","author":[{"given":"Patrick","family":"Schwarz","sequence":"first","affiliation":[{"name":"Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, CNRS FRE2849, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ste\u0301phane","family":"Bretagne","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, CNRS FRE2849, Institut Pasteur, Paris"},{"name":"Laboratoire de Parasitologie\u2014Mycologie, AP-HP, Ho\u0302pital Henri Mondor, Cre\u0301teil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Charles","family":"Gantier","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, CNRS FRE2849, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dea","family":"Garcia-Hermoso","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, CNRS FRE2849, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Lortholary","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, CNRS FRE2849, Institut Pasteur, Paris"},{"name":"Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine, AP-HP, Ho\u0302pital Necker-Enfants-Malades, Service des Maladies Infectieuses et Tropicales, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Dromer","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, CNRS FRE2849, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Dannaoui","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, CNRS FRE2849, Institut Pasteur, Paris"},{"name":"Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine, AP-HP, Ho\u0302pital Europe\u0301en Georges Pompidou, Unite\u0301 de Parasitologie\u2014Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.67.1725"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2656-2662.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.783-788.2003"},{"key":"e_1_3_2_5_2","unstructured":"Council of the European Communities. 1986. 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