{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T19:02:55Z","timestamp":1785178975941,"version":"3.55.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Trichomonas vaginalis<\/jats:italic>\n            PCR using reagents from a commercially available assay for\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            and\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            was evaluated for detection of infection in women and men attending a sexually transmitted disease clinic. Evaluations included three primer sets, endocervical swabs, vaginal swabs and urine, and various storage conditions. The TVK3\/TVK7 primer set was optimal in our hands with sensitivities ranging from 69.5 to 96.8%. In all comparisons,\n            <jats:italic>T. vaginalis<\/jats:italic>\n            PCR performed better than routine diagnostics using microscopy for women and culture for men (\n            <jats:italic>P<\/jats:italic>\n            &gt; 0.05). The assay performed well for all sample types tested, and vaginal swabs were stable for up to 7 days at ambient temperature. Using samples prepared for, and reagents from, the\n            <jats:italic>C. trachomatis-N. gonorrhoeae<\/jats:italic>\n            PCR assay allowed incorporation of\n            <jats:italic>T. vaginalis<\/jats:italic>\n            PCR diagnosis into routine clinical testing.\n          <\/jats:p>","DOI":"10.1128\/jcm.44.2.366-373.2006","type":"journal-article","created":{"date-parts":[[2006,2,2]],"date-time":"2006-02-02T18:33:16Z","timestamp":1138905196000},"page":"366-373","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Use of an Adaptation of a Commercially Available PCR Assay Aimed at Diagnosis of Chlamydia and Gonorrhea To Detect\n            <i>Trichomonas vaginalis<\/i>\n            in Urogenital Specimens"],"prefix":"10.1128","volume":"44","author":[{"given":"Barbara","family":"Van Der Pol","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colleen S.","family":"Kraft","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Emory University School of Medicine, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Williams","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200108004-00010"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000137900.63660.98"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199707000-00008"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.79.5.393"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00124256"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a116711"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.12.5619-5622.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199507000-00009"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199908000-00003"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.OLQ.0000079048.11771.46"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199605000-00011"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.1.89-95.2002"},{"key":"e_1_3_2_14_2","first-page":"819","volume":"40","year":"1994","unstructured":"Kengne, P., F. Veas, N. Vidal, J. L. Rey, and G. Cuny. 1994. Trichomonas vaginalis: repeated DNA target for highly sensitive and specific polymerase chain reaction diagnosis. Cell. Mol. Biol.40:819-831.","journal-title":"Cell. Mol. Biol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)93005-8"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199301000-00015"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.10.3585-3588.2000"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.11.3205-3210.1998"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2683-2687.2000"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/338399"},{"key":"e_1_3_2_21_2","first-page":"67","year":"1997","unstructured":"Orle, K. A., and J. B. Weiss. 1997. Detection of Treponema pallidum, Haemophilus ducreyi, and herpes simplex virus by multiplex PCR, p. 67-79. In R. W. Peeling and P. F. Sparling (ed.), Sexually transmitted diseases: methods and protocols. Humana Press, Totowa, New Jersey.","journal-title":"Sexually transmitted diseases: methods and protocols"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1067\/S0002-9378(03)00755-5"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199511000-00004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-200306000-00009"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.2.465-472.1992"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-200008000-00006"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1086\/376558"},{"key":"e_1_3_2_28_2","unstructured":"Arch. Pediatr. Adolesc. Med."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20257"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1001\/archpedi.155.6.676"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0706.010603"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(99)70509-0"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1105-1112.2000"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4127-4130.1999"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1080\/028418600431003"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1086\/319287"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1086\/427777"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1086\/342060"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(96)70268-5"},{"key":"e_1_3_2_40_2","unstructured":"World Health Organization. 2001. Global prevalence and incidence of selected curable sexually transmitted infections: overview and estimates. World Health Organization Geneva Switzerland."},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/1047-2797(94)00101-X"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.44.2.366-373.2006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.44.2.366-373.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T04:59:33Z","timestamp":1646456373000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.44.2.366-373.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,2]]}},"alternative-id":["10.1128\/JCM.44.2.366-373.2006"],"URL":"https:\/\/doi.org\/10.1128\/jcm.44.2.366-373.2006","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2]]},"assertion":[{"value":"2005-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-11-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}