{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:41:41Z","timestamp":1791096101118,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We identified\u2014by randomly amplified polymorphic DNA (RAPD) analysis at the population level followed by DNA differential display, cloning, and sequencing\u2014three prophage DNA fragments (F5, F7, and F10) in\n            <jats:italic>Streptococcus agalactiae<\/jats:italic>\n            that displayed significant sequence similarity to the DNA of\n            <jats:italic>S. agalactiae<\/jats:italic>\n            and\n            <jats:italic>Streptococcus pyogenes<\/jats:italic>\n            . The F5 sequence aligned with a prophagic gene encoding the large subunit of a terminase, F7 aligned with a phage-associated cell wall hydrolase and a phage-associated lysin, and F10 aligned with a transcriptional regulator (\n            <jats:italic>ArpU<\/jats:italic>\n            family) and a phage-associated endonuclease. We first determined the prevalence of F5, F7, and F10 by PCR in a collection of 109 strains isolated in the 1980s and divided into two populations: one with a high risk of causing meningitis (HR group) and the other with a lower risk of causing meningitis (LR group). These fragments were significantly more prevalent in the HR group than in the LR group (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). Our findings suggest that lysogeny has increased the ability of some\n            <jats:italic>S. agalactiae<\/jats:italic>\n            strains to invade the neonatal brain endothelium. We then determined the prevalence of F5, F7, and F10 by PCR in a collection of 40 strains recently isolated from neonatal meningitis cases for comparison with the cerebrospinal fluid (CSF) strains isolated in the 1980s. The prevalence of the three prophage DNA fragments was similar in these two populations isolated 15 years apart. We suggest that the prophage DNA fragments identified have remained stable in many CSF\n            <jats:italic>S. agalactiae<\/jats:italic>\n            strains, possibly due to their importance in virulence or fitness.\n          <\/jats:p>","DOI":"10.1128\/jcm.44.3.1049-1058.2006","type":"journal-article","created":{"date-parts":[[2006,3,3]],"date-time":"2006-03-03T22:28:19Z","timestamp":1141424899000},"page":"1049-1058","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Prophagic DNA Fragments in\n            <i>Streptococcus agalactiae<\/i>\n            Strains and Association with Neonatal Meningitis"],"prefix":"10.1128","volume":"44","author":[{"given":"Nathalie","family":"van der Mee-Marquet","sequence":"first","affiliation":[{"name":"Universite\u0301 Franc\u0327ois-Rabelais de Tours, IFR 136-EA 3854 \u201cBacte\u0301ries et risque maternofoetal,\u201d UFR Me\u0301decine, 2 bis, Boulevard Tonnele\u0301, 37032 Tours cedex, France"},{"name":"Service de Bacte\u0301riologie et Hygie\u0300ne, CHRU Trousseau, Centre National de Re\u0301fe\u0301rence pour Streptococcus agalactiae, 37044 Tours cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Sophie","family":"Domelier","sequence":"additional","affiliation":[{"name":"Universite\u0301 Franc\u0327ois-Rabelais de Tours, IFR 136-EA 3854 \u201cBacte\u0301ries et risque maternofoetal,\u201d UFR Me\u0301decine, 2 bis, Boulevard Tonnele\u0301, 37032 Tours cedex, France"},{"name":"Service de Bacte\u0301riologie et Hygie\u0300ne, CHRU Trousseau, Centre National de Re\u0301fe\u0301rence pour Streptococcus agalactiae, 37044 Tours cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Mereghetti","sequence":"additional","affiliation":[{"name":"Universite\u0301 Franc\u0327ois-Rabelais de Tours, IFR 136-EA 3854 \u201cBacte\u0301ries et risque maternofoetal,\u201d UFR Me\u0301decine, 2 bis, Boulevard Tonnele\u0301, 37032 Tours cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Lanotte","sequence":"additional","affiliation":[{"name":"Universite\u0301 Franc\u0327ois-Rabelais de Tours, IFR 136-EA 3854 \u201cBacte\u0301ries et risque maternofoetal,\u201d UFR Me\u0301decine, 2 bis, Boulevard Tonnele\u0301, 37032 Tours cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agne\u0300s","family":"Rosenau","sequence":"additional","affiliation":[{"name":"Universite\u0301 Franc\u0327ois-Rabelais de Tours, IFR 136-EA 3854 \u201cBacte\u0301ries et risque maternofoetal,\u201d UFR Me\u0301decine, 2 bis, Boulevard Tonnele\u0301, 37032 Tours cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem","family":"van Leeuwen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center Rotterdam, 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