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According to data from the Nebraska Veterinary Diagnostic Laboratory System, however,<jats:italic>Moraxella<\/jats:italic>(<jats:italic>Branhamella<\/jats:italic>)<jats:italic>ovis<\/jats:italic>has been isolated with increasing frequency from cattle affected with IBK. The objective of this study was, therefore, to examine<jats:italic>M. ovis<\/jats:italic>field isolates for the presence of the putative virulence factors of<jats:italic>M. bovis<\/jats:italic>. Culture filtrates from selected<jats:italic>M. ovis<\/jats:italic>field isolates demonstrated hemolytic activity on bovine erythrocytes and cytotoxic activity on bovine peripheral blood mononuclear cells and corneal epithelial cells. The hemolytic activity of the culture filtrates was attenuated after heat treatment. Polyclonal antibodies raised against the<jats:italic>M. bovis<\/jats:italic>hemolysin-cytotoxin also recognized a protein of approximately 98 kDa in a Western blot assay. These data indicate that the<jats:italic>M. ovis<\/jats:italic>field isolates examined produce one or more heat-labile exotoxins and may suggest that<jats:italic>M. ovis<\/jats:italic>plays a role in the pathogenesis of IBK.<\/jats:p>","DOI":"10.1128\/jcm.44.3.772-776.2006","type":"journal-article","created":{"date-parts":[[2006,3,3]],"date-time":"2006-03-03T22:28:19Z","timestamp":1141424899000},"page":"772-776","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Effects of<i>Moraxella<\/i>(<i>Branhamella<\/i>)<i>ovis<\/i>Culture Filtrates on Bovine Erythrocytes, Peripheral Mononuclear Cells, and Corneal Epithelial Cells"],"prefix":"10.1128","volume":"44","author":[{"given":"Henry E.","family":"Cerny","sequence":"first","affiliation":[{"name":"Crete Veterinary Clinic, Crete, Nebraska 68333-0008"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas G.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Nebraska\u2014Lincoln, Lincoln, Nebraska 68583-0907"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey T.","family":"Gray","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Guelph, Ontario Veterinary College, Guelph, Ontario, Canada N1G 2W1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Nebraska\u2014Lincoln, Lincoln, Nebraska 68583-0907"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Hinkley","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, University of Nebraska\u2014Lincoln, Lincoln, Nebraska 68583-0907"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00410-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.1222"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879000200116"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.11.4665-4672.1996"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(94)90074-4"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1994.tb07263.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3469-3474.2000"},{"key":"e_1_3_2_9_2","first-page":"157","year":"1988","unstructured":"Bisping, W., and G. 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