{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:03:11Z","timestamp":1777518191062,"version":"3.51.4"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In this paper we report on an experimental evaluation of phenotypic and molecular methods as means for the detection of oxacillin resistance in members of the\n            <jats:italic>Staphylococcus sciuri<\/jats:italic>\n            group. A total of 109\n            <jats:italic>S. sciuri<\/jats:italic>\n            group member isolates (92\n            <jats:italic>S. sciuri<\/jats:italic>\n            isolates, 9\n            <jats:italic>S. lentus<\/jats:italic>\n            isolates, and 8\n            <jats:italic>S. vitulinus<\/jats:italic>\n            isolates) were tested by the disk diffusion method, the agar dilution method, the oxacillin salt-agar screening method, slide latex agglutination for PBP 2a, and PCR assay for\n            <jats:italic>mecA<\/jats:italic>\n            as the reference method. The\n            <jats:italic>mecA<\/jats:italic>\n            gene was detected in 29\n            <jats:italic>S. sciuri<\/jats:italic>\n            isolates, and the true-positive and true-negative results of the other tests were defined on the basis of the presence or the absence of the\n            <jats:italic>mecA<\/jats:italic>\n            gene. For the different methods evaluated, the sensitivities and specificities were as follows: for the disk diffusion test with a 1-\u03bcg oxacillin disk, 100% and 55.9%, respectively; for the disk diffusion test with a 30-\u03bcg cefoxitin disk, 93.5% and 100%, respectively; for the agar dilution method, 100% and 50%, respectively; for the oxacillin salt-agar screen test (with 6 \u03bcg of oxacillin per ml and 4% NaCl) 100% and 100%, respectively; and for the slide latex agglutination test for PBP 2a, 100% and 100%, respectively. The disk diffusion test with various \u03b2-lactam antibiotics was performed to evaluate their use for the prediction of oxacillin resistance. The results indicate that meropenem, cefazolin, cefamandole, cefuroxime, cefotetan, cefoperazone, cefotaxime, ceftriaxone, moxalactam, cefaclor, and cefprozil may be used as surrogate markers of oxacillin resistance, although further studies of their use for the detection of oxacillin resistance are required.\n          <\/jats:p>","DOI":"10.1128\/jcm.44.3.934-937.2006","type":"journal-article","created":{"date-parts":[[2006,3,3]],"date-time":"2006-03-03T22:28:19Z","timestamp":1141424899000},"page":"934-937","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Evaluation of Phenotypic and Molecular Methods for Detection of Oxacillin Resistance in Members of the\n            <i>Staphylococcus sciuri<\/i>\n            Group"],"prefix":"10.1128","volume":"44","author":[{"given":"Srdjan","family":"Stepanovic\u0301","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"Hauschild","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biology, University of Bialystok, 15-950 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Dakic\u0301","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zainab","family":"Al-Doori","sequence":"additional","affiliation":[{"name":"Scottish MRSA Reference Laboratory, Microbiology Department, Stobhill Hospital, Glasgow G21 3UW, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milena","family":"S\u030cvabic\u0301-Vlahovic\u0301","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lazar","family":"Ranin","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald","family":"Morrison","sequence":"additional","affiliation":[{"name":"Scottish MRSA Reference Laboratory, Microbiology Department, Stobhill Hospital, Glasgow G21 3UW, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(03)00896-1"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/37.1.53"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.suppl_1.65"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/422458"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1136-1143.2000"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.2.645-653.2003"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2782-2785.2005"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2766-2771.2002"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3609-3614.2003"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/fmic.2001.0457"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.7.1768-1772.1994"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4401(02)00124-9"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2003.00662.x"},{"key":"e_1_3_1_15_2","first-page":"673","volume":"17","year":"1998","unstructured":"Hedin, G., and M. 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