{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:04:14Z","timestamp":1761764654913},"reference-count":28,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Group B\n            <jats:italic>Streptococcus<\/jats:italic>\n            (GBS;\n            <jats:italic>Streptococcus agalactiae<\/jats:italic>\n            ) is an important cause of sepsis and meningitis. Nine GBS serotypes, based on capsular polysaccharide (CPS) antigens, have been described. Their distribution varies worldwide and needs to be monitored to understand the epidemiology of GBS disease and inform the development of vaccines. In this study, we sequenced\n            <jats:italic>cpsH<\/jats:italic>\n            of GBS serotype II (\n            <jats:italic>cpsHII<\/jats:italic>\n            ) and compared it with that of the other eight serotypes to identify serotype-specific regions. We then developed a DNA microarray based on the\n            <jats:italic>cpsH<\/jats:italic>\n            gene and used it to test 88 GBS isolates\u20149 serotype reference strains and 79 clinical isolates\u2014and 7 other bacterial and fungal species which are commonly present in the vagina flora. The microarray was shown to be specific and reproducible. This is the first report of a microarray which can identify the nine GBS serotypes. The use of a microarray has advantages over traditional serotyping methods and will be of practical value in both reference and diagnostic laboratories.\n          <\/jats:p>","DOI":"10.1128\/jcm.44.4.1447-1452.2006","type":"journal-article","created":{"date-parts":[[2006,4,6]],"date-time":"2006-04-06T13:42:51Z","timestamp":1144330971000},"page":"1447-1452","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Use of a Serotype-Specific DNA Microarray for Identification of Group B\n            <i>Streptococcus<\/i>\n            (\n            <i>Streptococcus agalactiae<\/i>\n            )"],"prefix":"10.1128","volume":"44","author":[{"given":"Linyan","family":"Wen","sequence":"first","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"},{"name":"Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Wang","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"},{"name":"Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yayue","family":"Li","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"},{"name":"Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanrong","family":"Kong","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, 2145 New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwendolyn L.","family":"Gilbert","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, 2145 New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boyang","family":"Cao","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"},{"name":"Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"},{"name":"Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Feng","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"},{"name":"Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2564-2567.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.3.574"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.16.4466-4477.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.3096-3103.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1326-1329.2004"},{"key":"e_1_3_2_7_2","first-page":"594","volume":"84","year":"1974","unstructured":"Cropp, C. 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