{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T06:44:28Z","timestamp":1769237068346,"version":"3.49.0"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We developed a pair of primers that specifically identifies\n            <jats:italic>Coccidioides<\/jats:italic>\n            species, etiologic agents of the human fungal disease coccidioidomycosis. These primers could be used for distinguishing\n            <jats:italic>Coccidioides immitis<\/jats:italic>\n            and\n            <jats:italic>Coccidioides posadasii<\/jats:italic>\n            by simply comparing the amplicon sizes on an agarose gel.\n          <\/jats:p>","DOI":"10.1128\/jcm.44.5.1859-1862.2006","type":"journal-article","created":{"date-parts":[[2006,5,4]],"date-time":"2006-05-04T02:48:14Z","timestamp":1146710894000},"page":"1859-1862","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Novel Approach to Designing Primers for Identification and Distinction of the Human Pathogenic Fungi\n            <i>Coccidioides immitis<\/i>\n            and\n            <i>Coccidioides posadasii<\/i>\n            by PCR Amplification"],"prefix":"10.1128","volume":"44","author":[{"given":"Takashi","family":"Umeyama","sequence":"first","affiliation":[{"name":"Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Sano","sequence":"additional","affiliation":[{"name":"Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuhiko","family":"Kamei","sequence":"additional","affiliation":[{"name":"Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Niimi","sequence":"additional","affiliation":[{"name":"Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuko","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimasa","family":"Uehara","sequence":"additional","affiliation":[{"name":"Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1492-1493.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.778-783.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.071406098"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/15572536.2003.11833250"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/00275514.2000.12061175"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msh124"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.1982-1985.2004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.10.3505-3511.2001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.11.2913-2919.1995"},{"key":"e_1_3_2_11_2","unstructured":"Nippon Ishinkin Gakkai Zasshi (Jpn. 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