{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:46:37Z","timestamp":1791812797161,"version":"4.3.4"},"reference-count":9,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Microbiol Biol Educ."],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Hands-on lab experience is invaluable for students, not only for practicing foundational techniques but also for their application of the scientific process and scientific reasoning. Here, we describe a quantitative PCR (qPCR) teaching lab module that allows students to use their own standard-curve data to improve their technique and confidence in standard lab practices. We describe the module in the context of a\n                      <jats:italic toggle=\"yes\">Mesorhizobium loti<\/jats:italic>\n                      system, but the lab activities can be easily adapted to any other system in which (q)PCR is applicable. We then briefly describe extensions of this qPCR standard-curve module that can expand students\u2019 learning opportunities.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jmbe.00255-26","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:00:33Z","timestamp":1791810033000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Data-driven learning: a qPCR lab module that incorporates quantitative feedback to encourage technical problem solving"],"prefix":"10.1128","author":[{"given":"Nancy D.","family":"Shi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05rrcem69","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Agricultural and Environmental Sciences, UC Davis","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassie","family":"Tong-Zhou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05rrcem69","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Biological Sciences, UC Davis","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2015-5264","authenticated-orcid":true,"given":"Kenjiro W.","family":"Quides","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05rrcem69","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Biological Sciences, UC Davis","place":["Davis, USA"]},{"name":"Department of Microbiology and Molecular Genetics, UC Davis","place":["Davis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaf043"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsn017"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-134"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/biology12020277"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/bmb.21620"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jmbe.00125-21"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00572-020-01014-1"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jmbe.v18i2.1287"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jmbe.v21i3.2157"}],"container-title":["Journal of Microbiology &amp; Biology Education"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jmbe.00255-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jmbe.00255-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:00:36Z","timestamp":1791810036000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jmbe.00255-26"}},"subtitle":[],"editor":[{"given":"Stanley","family":"Maloy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,10,12]]},"references-count":9,"alternative-id":["10.1128\/jmbe.00255-26"],"URL":"https:\/\/doi.org\/10.1128\/jmbe.00255-26","relation":{},"ISSN":["1935-7877","1935-7885"],"issn-type":[{"value":"1935-7877","type":"print"},{"value":"1935-7885","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,12]]},"assertion":[{"value":"2026-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00255-26"}}