{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:26:39Z","timestamp":1793046399788,"version":"4.5.0"},"reference-count":8,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Microbiol Biol Educ."],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:p>Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.<\/jats:p>","DOI":"10.1128\/jmbe.v18i2.1348","type":"journal-article","created":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T12:30:21Z","timestamp":1506342621000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":116,"title":["Bacterial DNA Extraction Using Individual Enzymes and Phenol\/Chloroform Separation"],"prefix":"10.1128","volume":"18","author":[{"given":"Mitchell Henry","family":"Wright","sequence":"first","affiliation":[{"name":"Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health &amp; Science University, Portland, OR 97239-3098"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Adelskov","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, Griffith University, Nathan Campus, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony Carlson","family":"Greene","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, Griffith University, Nathan Campus, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.5530\/pj.2017.1.14"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00663-16"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16047493"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(61)80047-8"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-016-0408-8"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2008.02372.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.000802-0"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jmbe.v14i1.531"}],"container-title":["Journal of Microbiology &amp; Biology Education"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jmbe.v18i2.1348","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jmbe.v18i2.1348","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T21:15:37Z","timestamp":1646428537000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jmbe.v18i2.1348"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9]]},"references-count":8,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1128\/jmbe.v18i2.1348"],"URL":"https:\/\/doi.org\/10.1128\/jmbe.v18i2.1348","relation":{},"ISSN":["1935-7877","1935-7885"],"issn-type":[{"value":"1935-7877","type":"print"},{"value":"1935-7885","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9]]},"assertion":[{"value":"2017-05-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-07-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"18.2.60"}}