{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T22:26:45Z","timestamp":1788474405237,"version":"build-2803163510"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2011,7,15]],"date-time":"2011-07-15T00:00:00Z","timestamp":1310688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2011,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>In paramyxoviruses, the matrix (M) protein mediates the interaction between the envelope and internal proteins during particle assembly and egress. In measles virus (MeV), M mutations, such as those found in subacute sclerosing panencephalitis (SSPE) strains, and differences in vaccine and wild-type M proteins can affect the strength of interaction with the envelope glycoproteins, assembly efficiency, and spread. However, the contribution of the M protein to the replication and pathogenesis of the closely related canine distemper virus (CDV) has not been characterized. To this end this, we generated a recombinant wild-type CDV carrying a vaccine strain M protein. The recombinant virus retained the parental growth phenotype in VerodogSLAMtag cells, but displayed an increased particle-to-infectivity ratio very similar to that of the vaccine strain, likely due to inefficient H protein incorporation. Even though infectious virus was released only from the apical surface, consistent with the release polarity of the wild-type CDV strain, envelope protein distribution in polarized epithelial cells reproduced the bipolar pattern seen in vaccine strain-infected cells. Most notably, the chimeric virus was completely attenuated in ferrets and caused only a mild and transient leukopenia, indicating that the differences in particle infectivity and envelope protein sorting mediated by the vaccine M protein contribute importantly to vaccine strain attenuation.<\/jats:p>","DOI":"10.1128\/jvi.00051-11","type":"journal-article","created":{"date-parts":[[2011,5,5]],"date-time":"2011-05-05T03:33:06Z","timestamp":1304566386000},"page":"7162-7168","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Canine Distemper Virus Matrix Protein Influences Particle Infectivity, Particle Composition, and Envelope Distribution in Polarized Epithelial Cells and Modulates Virulence"],"prefix":"10.1128","volume":"85","author":[{"given":"Erik","family":"Dietzel","sequence":"first","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle E.","family":"Anderson","sequence":"additional","affiliation":[{"name":"INRS-Institut Armand-Frappier, University of Quebec, Laval, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Castan","sequence":"additional","affiliation":[{"name":"INRS-Institut Armand-Frappier, University of Quebec, Laval, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"von Messling","sequence":"additional","affiliation":[{"name":"INRS-Institut Armand-Frappier, University of Quebec, Laval, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Maisner","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01419-08"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1320"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.14.3899"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.2.1224-1234.1998"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-10-102"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030178"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/529515"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90359-4"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/520584"},{"key":"e_1_3_2_11_2","first-page":"1449","volume-title":"Fields virology","author":"Lamb R. 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