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The development of differentiated primary cultures of ferret nasal epithelial cells is an important step toward understanding cellular tropism and the mechanisms of influenza virus infection and replication in the airway milieu of this model. Using lectin staining and microscopy techniques, we characterized the sialic acid receptor distribution and the cellular composition of the culture model. We then evaluated the replication of and immune response to human and avian influenza viruses at relevant physiological temperatures. Our findings offer significant insight into this first line of defense against influenza virus infection and provide a model for the evaluation of emerging influenza viruses in a well-controlled\n                    <jats:italic>in vitro<\/jats:italic>\n                    environmental setting.\n                  <\/jats:p>","DOI":"10.1128\/jvi.00080-19","type":"journal-article","created":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T05:48:46Z","timestamp":1551246526000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Tropism and Infectivity of a Seasonal A(H1N1) and a Highly Pathogenic Avian A(H5N1) Influenza Virus in Primary Differentiated Ferret Nasal Epithelial Cell Cultures"],"prefix":"10.1128","volume":"93","author":[{"given":"Hui","family":"Zeng","sequence":"first","affiliation":[{"name":"Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and 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