{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:51:30Z","timestamp":1792511490590,"version":"4.5.0"},"reference-count":84,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T00:00:00Z","timestamp":1721692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T00:00:00Z","timestamp":1721692800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["OCENW.M.21.073"],"award-info":[{"award-number":["OCENW.M.21.073"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2024,7,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Live-attenuated flavivirus vaccines confer long-term protection against disease, but the design of attenuated flaviviruses does not follow a general approach. The non-coding, subgenomic flavivirus RNA (sfRNA) is produced by all flaviviruses and is an essential factor in viral pathogenesis and transmission. We argue that modulating sfRNA expression is a promising, universal strategy to finetune flavivirus attenuation for developing effective flavivirus vaccines of the future.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.00100-23","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T09:02:24Z","timestamp":1716973344000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Subgenomic flavivirus RNA as key target for live-attenuated vaccine development"],"prefix":"10.1128","volume":"98","author":[{"given":"Kristel","family":"Doets","sequence":"first","affiliation":[{"name":"Wageningen University and Research, Laboratory of Virology, Wageningen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9301-0408","authenticated-orcid":true,"given":"Gorben P.","family":"Pijlman","sequence":"additional","affiliation":[{"name":"Wageningen University and Research, Laboratory of Virology, Wageningen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0714-0"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.08347"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0508391103"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-8727-1_1"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011880"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585(2006)43[623:rbhvav]2.0.co;2"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1941"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2016.11.005"},{"key":"e_1_3_1_11_2","volume-title":"Fundamentals of Molecular Virology","author":"Acheson NH.","year":"2011","unstructured":"Acheson NH. 2011. Fundamentals of Molecular Virology, 2. John Wiley & Sons Inc.","edition":"2"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0065-3527(08)60316-4"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1980.29.643"},{"key":"e_1_3_1_14_2","volume-title":"Flaviviridae: the viruses and their replicationFields Virology","author":"Lindenbach BD","year":"2007","unstructured":"Lindenbach BD, Thiel H-J, Rice CM. 2007. Flaviviridae: the viruses and their replicationFields Virology, 5. Lippincott-Raven, Philadelphia.","edition":"5"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.11.6631-6643.2005"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.08.004"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2008.10.007"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1905617116"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1109"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01159-10"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006265"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab462"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28977-3"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01047-10"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.034330.112"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.009129"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00207-12"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.04.087"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.add8095"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2018.09.006"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00930-16"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00343-20"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16086-y"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2301790"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2022.2046960"},{"key":"e_1_3_1_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005584"},{"key":"e_1_3_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.07.067"},{"key":"e_1_3_1_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00172-08"},{"key":"e_1_3_1_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.06.014"},{"key":"e_1_3_1_40_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2010.0179"},{"key":"e_1_3_1_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.3.2132-2140.1998"},{"key":"e_1_3_1_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0042-6822(03)00450-1"},{"key":"e_1_3_1_43_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2005.5.1"},{"key":"e_1_3_1_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.06.008"},{"key":"e_1_3_1_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.07.064"},{"key":"e_1_3_1_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw501"},{"key":"e_1_3_1_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4322"},{"key":"e_1_3_1_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00737-8"},{"key":"e_1_3_1_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13589-1"},{"key":"e_1_3_1_50_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.39023"},{"key":"e_1_3_1_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01108-23"},{"key":"e_1_3_1_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/v12101177"},{"key":"e_1_3_1_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60238-X"},{"key":"e_1_3_1_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12012"},{"key":"e_1_3_1_55_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1800820"},{"key":"e_1_3_1_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30494-8"},{"key":"e_1_3_1_57_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1506223"},{"key":"e_1_3_1_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.21.11436-11447.2003"},{"key":"e_1_3_1_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.70.6.3930-3937.1996"},{"key":"e_1_3_1_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.2.1653-1657.2003"},{"key":"e_1_3_1_61_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2004.71.811"},{"key":"e_1_3_1_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.11.082"},{"key":"e_1_3_1_63_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-4-39"},{"key":"e_1_3_1_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0264-410x(03)00488-2"},{"key":"e_1_3_1_65_2","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2006.19.10"},{"key":"e_1_3_1_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.07.023"},{"key":"e_1_3_1_67_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.2.4.2944"},{"key":"e_1_3_1_68_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.2.6.3494"},{"key":"e_1_3_1_69_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.405"},{"key":"e_1_3_1_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.05.075"},{"key":"e_1_3_1_71_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001267"},{"key":"e_1_3_1_72_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis936"},{"key":"e_1_3_1_73_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv082"},{"key":"e_1_3_1_74_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaf1517"},{"key":"e_1_3_1_75_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw067"},{"key":"e_1_3_1_76_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.414"},{"key":"e_1_3_1_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30023-2"},{"key":"e_1_3_1_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2013.09.016"},{"key":"e_1_3_1_79_2","doi-asserted-by":"publisher","DOI":"10.3390\/v6020404"},{"key":"e_1_3_1_80_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofac492.1758"},{"key":"e_1_3_1_81_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.156752"},{"key":"e_1_3_1_82_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009788"},{"key":"e_1_3_1_83_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004604"},{"key":"e_1_3_1_84_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2015.1094599"},{"key":"e_1_3_1_85_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2021.2016354"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.00100-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.00100-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T09:21:39Z","timestamp":1721726499000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.00100-23"}},"subtitle":[],"editor":[{"given":"Suchetana","family":"Mukhopadhyay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,7,23]]},"references-count":84,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7,23]]}},"alternative-id":["10.1128\/jvi.00100-23"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00100-23","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,23]]},"assertion":[{"value":"2024-05-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00100-23"}}