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An adoptive transfer model was used to elucidate the kinetics of CD8<jats:sup>+<\/jats:sup>T-cell priming during CNS infection with RJHM as well as with two MHV strains that induce a robust CD8<jats:sup>+<\/jats:sup>T-cell response (RA59 and SJHM\/RA59, a recombinant A59 virus expressing the JHM spike). While RA59 and SJHM\/RA59 infections resulted in CD8<jats:sup>+<\/jats:sup>T-cell priming within the first 2 days postinfection, RJHM infection did not lead to proliferation of nai\u0308ve CD8<jats:sup>+<\/jats:sup>T cells. While all three viruses replicated efficiently in the brain, only RA59 and SJHM\/RA59 replicated to appreciable levels in the cervical lymph nodes (CLN), the site of T-cell priming during acute CNS infection. RJHM was unable to suppress the CD8<jats:sup>+<\/jats:sup>T-cell response elicited by RA59 in mice simultaneously infected with both strains, suggesting that RJHM does not cause generalized immunosuppression. RJHM was also unable to elicit a secondary CD8<jats:sup>+<\/jats:sup>T-cell response in the brain following peripheral immunization against a viral epitope. Notably, the weak CD8<jats:sup>+<\/jats:sup>T-cell response elicited by RJHM was unique to CNS infection, since peripheral inoculation induced a robust CD8<jats:sup>+<\/jats:sup>T-cell response in the spleen. These findings suggest that the failure of RJHM to prime a robust CD8<jats:sup>+<\/jats:sup>T-cell response during CNS infection is likely due to its failure to replicate in the CLN.<\/jats:p>","DOI":"10.1128\/jvi.00106-08","type":"journal-article","created":{"date-parts":[[2008,4,17]],"date-time":"2008-04-17T01:10:29Z","timestamp":1208394629000},"page":"6150-6160","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Priming of CD8<sup>+<\/sup>T Cells during Central Nervous System Infection with a Murine Coronavirus Is Strain Dependent"],"prefix":"10.1128","volume":"82","author":[{"given":"Katherine C.","family":"MacNamara","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan J.","family":"Bender","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming Ming","family":"Chua","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Watson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan R.","family":"Weiss","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01321116"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1477"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.6.3204"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210930"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(84)90033-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.3.1150-1159.2004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.59.2.463-471.1986"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90306-A"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.10.6541-6547.1995"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0882-4010(95)90058-6"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(92)90215-7"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1557"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1156"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00432-06"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.4.2353"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.5.2919"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.34.5.597"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(87)90066-0"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(03)00249-2"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.6.3370-3381.2005"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.14.9108-9118.2005"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-1325-4_52"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.4.2775-2778.2003"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1202"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.5.3063-3070.2005"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80470-7"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.8.4972-4978.2003"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.19.10260-10269.2003"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.4049\/jimmunol.162.3.1641","volume":"162","year":"1999","unstructured":"Parra, B., D. 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