{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:07:13Z","timestamp":1791407233942,"version":"4.3.3"},"reference-count":72,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2010,7,15]],"date-time":"2010-07-15T00:00:00Z","timestamp":1279152000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Respiratory syncytial virus (RSV) is the etiological agent of acute respiratory diseases, such as bronchiolitis and pneumonia. The exacerbated production of proinflammatory cytokines and chemokines in the airways in response to RSV is an important pillar in the development of these pathologies. As such, a keen understanding of the mechanisms that modulate the inflammatory response during RSV infection is of pivotal importance to developing effective treatment. The NF-\u03baB transcription factor is a major regulator of proinflammatory cytokine and chemokine genes. However, RSV-mediated activation of NF-\u03baB is far from characterized. We recently demonstrated that aside from the well-characterized I\u03baB\u03b1 phosphorylation and degradation, the phosphorylation of p65 at Ser536 is an essential event regulating the RSV-mediated NF-\u03baB-dependent promoter transactivation. In the present study, using small interfering RNA and pharmacological inhibitors, we now demonstrate that RSV sensing by the RIG-I cytoplasmic receptor triggers a signaling cascade involving the MAVS and TRAF6 adaptors that ultimately leads to p65ser536 phosphorylation by the IKK\u03b2 kinase. In a previous study, we highlighted a critical role of the NOX2-containing NADPH oxidase enzyme as an upstream regulator of both the I\u03baB\u03b1Ser32 and p65Ser536 in human airway epithelial cells. Here, we demonstrate that inhibition of NOX2 significantly decreases IKK\u03b2 activation. Taken together, our data identify a new RIG-I\/MAVS\/TRAF6\/IKK\u03b2\/p65Ser536 pathway placed under the control of NOX2, thus characterizing a novel regulatory pathway involved in NF-\u03baB-driven proinflammatory response in the context of RSV infection.<\/jats:p>","DOI":"10.1128\/jvi.00142-10","type":"journal-article","created":{"date-parts":[[2010,4,22]],"date-time":"2010-04-22T02:12:32Z","timestamp":1271902352000},"page":"7267-7277","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":113,"title":["Respiratory Syncytial Virus-Mediated NF-\u03baB p65 Phosphorylation at Serine 536 Is Dependent on RIG-I, TRAF6, and IKK\u03b2"],"prefix":"10.1128","volume":"84","author":[{"given":"Fabrice","family":"Yoboua","sequence":"first","affiliation":[{"name":"CRCHUM-Centre Hospitalier de l'Universite\u0301 de Montre\u0301al, Montre\u0301al, Quebec H2X 1P1, Canada"},{"name":"Department of Biochemistry, Faculty of Medicine, Universite\u0301 de Montre\u0301al, Montre\u0301al, Quebec H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"Martel","sequence":"additional","affiliation":[{"name":"CRCHUM-Centre Hospitalier de l'Universite\u0301 de Montre\u0301al, Montre\u0301al, Quebec H2X 1P1, Canada"},{"name":"Department of Biochemistry, Faculty of Medicine, Universite\u0301 de Montre\u0301al, Montre\u0301al, Quebec H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annick","family":"Duval","sequence":"additional","affiliation":[{"name":"CRCHUM-Centre Hospitalier de l'Universite\u0301 de Montre\u0301al, Montre\u0301al, Quebec H2X 1P1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Espe\u0301rance","family":"Mukawera","sequence":"additional","affiliation":[{"name":"CRCHUM-Centre Hospitalier de l'Universite\u0301 de Montre\u0301al, Montre\u0301al, Quebec H2X 1P1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Grandvaux","sequence":"additional","affiliation":[{"name":"CRCHUM-Centre Hospitalier de l'Universite\u0301 de Montre\u0301al, Montre\u0301al, Quebec H2X 1P1, Canada"},{"name":"Department of Biochemistry, Faculty of Medicine, Universite\u0301 de Montre\u0301al, Montre\u0301al, Quebec H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01659-08"},{"key":"e_1_3_2_3_2","first-page":"129","volume":"124","year":"2009","unstructured":"Barral, P. 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