{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:01:50Z","timestamp":1790424110202,"version":"4.1.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            West Nile virus (WNV) infection leads to rapid and sustained Ca\n            <jats:sup>2+<\/jats:sup>\n            influx. This influx was observed with different strains of WNV and in different types of cells. Entry during virion endocytosis as well as through calcium channels contributed to the Ca\n            <jats:sup>2+<\/jats:sup>\n            influx observed in WNV-infected cells. Ca\n            <jats:sup>2+<\/jats:sup>\n            influx was not detected after infection with vesicular stomatitis virus (VSV) and occurred only through endocytosis in Sindbis virus-infected cells. Caspase 3 cleavage and activation of several kinases, including focal adhesion kinase (FAK), mitogen-activated extracellular signal-regulated protein kinase (ERK1\/2), and protein-serine kinase B alpha (Akt), at early times after WNV infection were shown to be dependent on Ca\n            <jats:sup>2+<\/jats:sup>\n            influx. Although the activation of these kinases was sustained in virus-infected cells throughout infection, UV-inactivated WNV induced only a transient activation of FAK and ERK1\/2 at early times after infection. The Ca\n            <jats:sup>2+<\/jats:sup>\n            -dependent FAK activation observed in WNV-infected cells was not mediated by \u03b1v\u03b23 integrins. Reduction of Ca\n            <jats:sup>2+<\/jats:sup>\n            influx at early times of infection by various treatments decreased the viral yield and delayed both the early transient caspase 3 cleavage and the activation of FAK, Akt, and ERK signaling. The results indicate that Ca\n            <jats:sup>2+<\/jats:sup>\n            influx is required for early infection events needed for efficient viral replication, possibly for virus-induced rearrangement of the endoplasmic reticulum (ER) membrane. Increased caspase 3 cleavage at both early (transient) and late times of infection correlated with decreased activation of the FAK and ERK1\/2 pathways, indicating a role for these kinases in extending the survival of flavivirus-infected cells.\n          <\/jats:p>","DOI":"10.1128\/jvi.00144-10","type":"journal-article","created":{"date-parts":[[2010,6,11]],"date-time":"2010-06-11T02:17:16Z","timestamp":1276222636000},"page":"8721-8731","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":87,"title":["Virus-Induced Ca\n            <sup>2+<\/sup>\n            Influx Extends Survival of West Nile Virus-Infected Cells"],"prefix":"10.1128","volume":"84","author":[{"given":"Svetlana V.","family":"Scherbik","sequence":"first","affiliation":[{"name":"Department of Biology, Georgia State University, Atlanta, Georgia 30302"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margo A.","family":"Brinton","sequence":"additional","affiliation":[{"name":"Department of Biology, Georgia State University, Atlanta, Georgia 30302"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007186200"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8705"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.5.597"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1372"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1155"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.294.5550.2376"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032427999"},{"key":"e_1_3_2_9_2","first-page":"1001","volume":"14","year":"1999","unstructured":"Cary, L. A., D. C. Han, and J. L. Guan. 1999. Integrin-mediated signal transduction pathways. Histol. Histopathol.14:1001-1009.","journal-title":"Histol. Histopathol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.10.019"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.09.025"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200301084"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.19.10543-10555.2004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M410208200"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.10.1793"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.3.1290-1301.2006"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374372"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.109.6.1615"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(07)00565-9"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404054200"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(97)90008-1"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.1.C11"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(95)00277-6"},{"key":"e_1_3_2_24_2","first-page":"17","volume":"7","year":"1990","unstructured":"Kielian, M., and S. Jungerwirth. 1990. Mechanisms of enveloped virus entry into cells. Mol. Biol. Med.7:17-31.","journal-title":"Mol. Biol. Med."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02210-06"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.13.8388-8399.2005"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.5.C1526"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19496-0"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090521697"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01165.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0351088"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.3109\/09687689609160598"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00008-08"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3320281"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060208-104626"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.015255-0"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.1.226-235.2006"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2009.02.009"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(02)00195-1"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.24.15007-15015.2005"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007109008111"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.14.3.1680-1688.1994"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.6.2987-2999.2006"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01359-07"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6107(98)00052-2"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.1.460-469.2003"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.15.10566"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(98)01243-7"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.105.1.191"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.2174\/138161206775474503"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4652(200009)184:3<385::AID-JCP14>3.0.CO;2-7"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.04.007"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.07.001"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(02)00142-0"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0795"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2009.05.005"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00144-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00144-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:58:11Z","timestamp":1646485091000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00144-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":55,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1128\/JVI.00144-10"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00144-10","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9]]},"assertion":[{"value":"2010-01-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-06-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}