{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:37:23Z","timestamp":1791841043434,"version":"4.3.4"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T00:00:00Z","timestamp":1713225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T00:00:00Z","timestamp":1713225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32002248"],"award-info":[{"award-number":["32002248"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"\u5c71\u4e1c\u7701\u79d1\u5b66\u6280\u672f\u5385 | Natural Science Foundation of Shandong Province","award":["ZR2020QC016"],"award-info":[{"award-number":["ZR2020QC016"]}]},{"name":"\u5c71\u4e1c\u7701\u79d1\u5b66\u6280\u672f\u5385 | Natural Science Foundation of Shandong Province","award":["ZR2020QC017"],"award-info":[{"award-number":["ZR2020QC017"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2024,4,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Equine herpesvirus type 8 (EHV-8) causes abortion and respiratory disease in horses and donkeys, leading to serious economic losses in the global equine industry. Currently, there is no effective vaccine or drug against EHV-8 infection, underscoring the need for a novel antiviral drug to prevent EHV-8-induced latent infection and decrease the pathogenicity of this virus. The present study demonstrated that hyperoside can exert antiviral effects against EHV-8 infection in RK-13 (rabbit kidney cells), MDBK (Madin\u2013Darby bovine kidney), and NBL-6 cells (E. Derm cells). Mechanistic investigations revealed that hyperoside induces heme oxygenase-1 expression by activating the c-Jun N-terminal kinase\/nuclear factor erythroid-2-related factor 2\/Kelch-like ECH-associated protein 1 axis, alleviating oxidative stress and triggering a downstream antiviral interferon response. Accordingly, hyperoside inhibits EHV-8 infection. Meanwhile, hyperoside can also mitigate EHV-8-induced injury in the lungs of infected mice. These results indicate that hyperoside may serve as a novel antiviral agent against EHV-8 infection.<\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Hyperoside has been reported to suppress viral infections, including herpesvirus, hepatitis B virus, infectious bronchitis virus, and severe acute respiratory syndrome coronavirus 2 infection. However, its mechanism of action against equine herpesvirus type 8 (EHV-8) is currently unknown. Here, we demonstrated that hyperoside significantly inhibits EHV-8 adsorption and internalization in susceptible cells. This process induces HO-1 expression via c-Jun N-terminal kinase\/nuclear factor erythroid-2-related factor 2\/Kelch-like ECH-associated protein 1 axis activation, alleviating oxidative stress and triggering an antiviral interferon response. These findings indicate that hyperoside could be very effective as a drug against EHV-8.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.00159-24","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T00:00:30Z","timestamp":1710806430000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Hyperoside inhibits EHV-8 infection via alleviating oxidative stress and IFN production through activating JNK\/Keap1\/Nrf2\/HO-1 signaling pathways"],"prefix":"10.1128","volume":"98","author":[{"given":"Tongtong","family":"Wang","sequence":"first","affiliation":[{"name":"College of Agronomy, Liaocheng University, Liaocheng, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyu","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Liaocheng University, Liaocheng, China"},{"name":"College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruibo","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agronomy, Liaocheng University, Liaocheng, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiying","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuwen","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agronomy, Liaocheng University, Liaocheng, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Agronomy, Liaocheng University, Liaocheng, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangdan","family":"Ding","sequence":"additional","affiliation":[{"name":"College of Agronomy, Liaocheng University, Liaocheng, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8533-5015","authenticated-orcid":false,"given":"Yubao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agronomy, Liaocheng University, Liaocheng, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5186-1703","authenticated-orcid":false,"given":"Changfa","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Liaocheng University, Liaocheng, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5431-9103","authenticated-orcid":true,"given":"Liangliang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agronomy, Liaocheng University, Liaocheng, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.840754"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-023-05704-x"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00445-12"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-021-01738-2"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-019-1125-9"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-020-00310-6"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01863-21"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2017.01.003"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-014-9989-8"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21010131"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-021-03404-z"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.15430\/JCP.2016.21.1.41"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01272"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.31.743"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.07.013"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2007.00510.x"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2020.153315"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20235832"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2018.8948"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-018-1151-x"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2020.8065"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2018.11.016"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3344"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.01.033"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2018.02.010"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21134777"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani12192495"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9101014"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2016.7"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.7314\/apjcp.2012.13.8.3653"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.765553"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.673509"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02668"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24054673"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-019-0679-2"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13102090"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0421-x"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01342-19"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601414"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112545"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2217"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.785057"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.9b01865"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.04.027"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10050789"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23160"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2021.06.023"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2017.09.010"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.06.046"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.865343"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/v12010040"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00979-19"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.1177\/03009858211020670"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.00159-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.00159-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T11:57:19Z","timestamp":1713268639000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.00159-24"}},"subtitle":[],"editor":[{"given":"Jae U.","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,4,16]]},"references-count":53,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4,16]]}},"alternative-id":["10.1128\/jvi.00159-24"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00159-24","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,16]]},"assertion":[{"value":"2024-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00159-24"}}