{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:40:21Z","timestamp":1773967221445,"version":"3.50.1"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["75N93019C00052"],"award-info":[{"award-number":["75N93019C00052"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"publisher","award":["France 2030\/ANR-23-IHUA-0009"],"award-info":[{"award-number":["France 2030\/ANR-23-IHUA-0009"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2025,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Domestic ferrets (\n                      <jats:italic>Mustela putorius furo<\/jats:italic>\n                      ) are important for modeling human respiratory diseases. However, ferret B and T cell receptors have not been completely identified or annotated, limiting immune repertoire studies. Here, we performed long-read transcriptome sequencing of ferret splenocyte and lymph node samples to obtain over 120,000 high-quality full-length immunoglobin (Ig) and T cell receptor (TCR) transcripts. We constructed a complete reference set of the constant regions of ferret Ig and TCR isotypes and chain types. We also systematically annotated germline Ig and TCR variable (V), diversity (D), joining (J), and constant (C) genes on a recent ferret reference genome assembly. We designed new ferret-specific immune repertoire profiling assays by targeting positions in constant regions without allelic diversity across 11 ferret genome assemblies and experimentally validated them using a commercially compatible single-cell-based platform. These improved resources and assays will enable future studies to fully capture ferret immune repertoire diversity.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Domestic ferrets (\n                        <jats:italic>Mustela putorius furo<\/jats:italic>\n                        ) are an increasingly common model organism to study human respiratory diseases such as influenza infections. However, researchers lack ferret-specific reagents and resources to study the immune system and immune response in ferrets. In this study, we developed comprehensive ferret immune repertoire reference resources and assays, which will enable more accurate analyses of the ferret immune system in the future.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.00181-25","type":"journal-article","created":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T09:35:44Z","timestamp":1742549744000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Development of ferret immune repertoire reference resources and single-cell-based high-throughput profiling assays"],"prefix":"10.1128","volume":"99","author":[{"given":"Evan S.","family":"Walsh","sequence":"first","affiliation":[{"name":"Department of Molecular Biomedical Sciences, North Carolina State University College of Veterinary Medicine","place":["Raleigh, USA"]},{"name":"Bioinformatics Graduate Program, North Carolina 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N.","family":"Brochu","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedical Sciences, North Carolina State University College of Veterinary Medicine","place":["Raleigh, USA"]},{"name":"Bioinformatics Graduate Program, North Carolina State University","place":["Raleigh, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsuan","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedical Sciences, North Carolina State University College of Veterinary Medicine","place":["Raleigh, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2482-0022","authenticated-orcid":false,"given":"Sofia","family":"Kossida","sequence":"additional","affiliation":[{"name":"IMGT, The International ImMunoGeneTics Information System, Institute of Human Genetics, National Center for Scientific Research (CNRS), University of Montpellier (UM)","place":["Montpellier, 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