{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T08:02:00Z","timestamp":1781596920733,"version":"3.54.5"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T00:00:00Z","timestamp":1316044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2011,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Monkeypox virus (MPXV) is an orthopoxvirus closely related to variola virus, the causative agent of smallpox. Human MPXV infection results in a disease that is similar to smallpox and can also be fatal. Two clades of MPXV have been identified, with viruses of the central African clade displaying more pathogenic properties than those within the west African clade. The monkeypox inhibitor of complement enzymes (MOPICE), which is not expressed by viruses of the west African clade, has been hypothesized to be a main virulence factor responsible for increased pathogenic properties of central African strains of MPXV. To gain a better understanding of the role of MOPICE during MPXV-mediated disease, we compared the host adaptive immune response and disease severity following intrabronchial infection with MPXV-Zaire (\n            <jats:italic>n<\/jats:italic>\n            = 4), or a recombinant MPXV-Zaire (\n            <jats:italic>n<\/jats:italic>\n            = 4) lacking expression of MOPICE in rhesus macaques (RM). Data presented here demonstrate that infection of RM with MPXV leads to significant viral replication in the peripheral blood and lungs and results in the induction of a robust and sustained adaptive immune response against the virus. More importantly, we show that the loss of MOPICE expression results in enhanced viral replication\n            <jats:italic>in vivo<\/jats:italic>\n            , as well as a dampened adaptive immune response against MPXV. Taken together, these findings suggest that MOPICE modulates the anti-MPXV immune response and that this protein is not the sole virulence factor of the central African clade of MPXV.\n          <\/jats:p>","DOI":"10.1128\/jvi.00199-11","type":"journal-article","created":{"date-parts":[[2011,7,14]],"date-time":"2011-07-14T01:51:28Z","timestamp":1310608288000},"page":"9527-9542","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":87,"title":["Deletion of the Monkeypox Virus Inhibitor of Complement Enzymes Locus Impacts the Adaptive Immune Response to Monkeypox Virus in a Nonhuman Primate Model of Infection"],"prefix":"10.1128","volume":"85","author":[{"given":"Ryan D.","family":"Estep","sequence":"first","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilhem","family":"Messaoudi","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"},{"name":"Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan A.","family":"O'Connor","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Li","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerald","family":"Sprague","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Barron","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flora","family":"Engelmann","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonnie","family":"Yen","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael F.","family":"Powers","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Jones","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bridget A.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata U.","family":"Orzechowska","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minsha","family":"Manoharan","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred","family":"Legasse","sequence":"additional","affiliation":[{"name":"Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"Planer","sequence":"additional","affiliation":[{"name":"Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Wilk","sequence":"additional","affiliation":[{"name":"Division of Animal Resources, Oregon National Primate Research Center, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael K.","family":"Axthelm","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"},{"name":"Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott W.","family":"Wong","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health &amp; Science University, Beaverton, Oregon"},{"name":"Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2006.00392.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1679"},{"key":"e_1_3_3_4_2","first-page":"165","article-title":"Human monkeypox, 1970\u201379","volume":"58","author":"Breman J. G.","year":"1980","unstructured":"BremanJ. G.. 1980. Human monkeypox, 1970\u201379. Bull. World Health Organ. 58:165\u2013182.","journal-title":"Bull. World Health Organ."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-96"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.03.037"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1113"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.05.030"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.05.008"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00856-9"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01474-10"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm917"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a011720"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0703.017311"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.005108-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.2.628"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01931-10"},{"key":"e_1_3_3_18_2","first-page":"747","article-title":"Monkeypox virus: ecology and public health significance","volume":"66","author":"Khodakevich L.","year":"1988","unstructured":"KhodakevichL. JezekZ. MessingerD.. 1988. Monkeypox virus: ecology and public health significance. Bull. World Health Organ. 66:747\u2013752.","journal-title":"Bull. World Health Organ."},{"key":"e_1_3_3_19_2","first-page":"115","article-title":"The role of squirrels in sustaining monkeypox virus transmission","volume":"39","author":"Khodakevich L.","year":"1987","unstructured":"KhodakevichL.. 1987. The role of squirrels in sustaining monkeypox virus transmission. Trop. Geogr. Med. 39:115\u2013122.","journal-title":"Trop. Geogr. Med."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2006.06.016"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1824"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81215-0"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.6.3725"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr070432"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1655"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000657"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.8396"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.1.29"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032299"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.010207-0"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.155"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022324511384"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1446"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.12.7845-7851.2005"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-7-150"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(00)00210-0"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00691.x"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200704-046AW"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780373"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00199-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00199-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T16:12:21Z","timestamp":1646496741000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00199-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,15]]},"references-count":38,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2011,9,15]]}},"alternative-id":["10.1128\/JVI.00199-11"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00199-11","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,15]]},"assertion":[{"value":"2011-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-06-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-08-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}