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However, except for the EV71 vaccine on the market, there are no effective strategies to prevent and treat other EV pathogen infections. Therefore, broad-spectrum anti-EV drugs are urgently needed. In this study, we demonstrated that FNC, a small nucleoside analog inhibitor that has been demonstrated to be a potent inhibitor of HIV and entered into a clinical phase II trial in China, potently inhibits the viral replication of a multitude of EVs at the nanomolar level. Further investigation revealed that FNC inhibits positive- and negative-strand RNA synthesis of EVs by interacting and interfering with the activity of EV71 viral RNA-dependent RNA polymerase (3D\n            <jats:sup>pol<\/jats:sup>\n            ). Our findings demonstrate for the first time that FNC is an effective broad-spectrum inhibitor for human EV pathogens.\n          <\/jats:p>","DOI":"10.1128\/jvi.00204-20","type":"journal-article","created":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T12:13:47Z","timestamp":1582114427000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["The Pyrimidine Analog FNC Potently Inhibits the Replication of Multiple Enteroviruses"],"prefix":"10.1128","volume":"94","author":[{"given":"Na","family":"Xu","sequence":"first","affiliation":[{"name":"Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baisong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Cao","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Huan","sequence":"additional","affiliation":[{"name":"Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junbiao","family":"Chang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2013.09.020"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0005-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-019-1192-8"},{"key":"e_1_3_2_5_2","volume-title":"Hand foot and mouth disease","author":"Guerra AM","year":"2019","unstructured":"Guerra AM, Waseem M. 2019. 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