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Influenza virus polymerase, the molecule that copies the viral RNA genome, hijacks cellular proteins to support its replication. Current anti-influenza drugs are aimed against viral proteins, including the polymerase, but RNA viruses like influenza tend to become resistant to such drugs very rapidly. An alternative strategy is to design therapeutics that target the host proteins that are necessary for virus propagation. Here, we show that the human proteins ANP32A and ANP32B are essential for influenza A and B virus replication, such that in their absence cells become impervious to the virus. We map the proviral activity of ANP32 proteins to one region in particular, which could inform future intervention.<\/jats:p>","DOI":"10.1128\/jvi.00217-19","type":"journal-article","created":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T07:00:46Z","timestamp":1560754846000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["ANP32 Proteins Are Essential for Influenza Virus Replication in Human Cells"],"prefix":"10.1128","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8443-5559","authenticated-orcid":false,"given":"Ecco","family":"Staller","sequence":"first","affiliation":[{"name":"Section of Molecular Virology, Imperial College London, St Mary\u2019s Campus, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3285-3804","authenticated-orcid":false,"given":"Carol 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