{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T17:10:21Z","timestamp":1780333821993,"version":"3.54.1"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Following virus infection, one of the cellular responses to limit the virus spread is induction of apoptosis. In the present study, we report role of rotavirus nonstructural protein 1 (NSP1) in regulating apoptosis by activating prosurvival pathways such as phosphatidylinositol 3-kinase (PI3K)\/Akt and NF-\u03baB (nuclear factor \u03baB) during early hours of infections (2 to 8 hpi). The NSP1 mutant strain A5-16 induces weak and transient activation of Akt (protein kinase B) and p65 NF-\u03baB compared to the isogenic wild-type strain A5-13 in MA104 or HT29 cells. The weak NF-\u03baB promoter activity or Akt phosphorylation after A5-16 infection could be complemented in cells transfected with plasmid expressing NSP1 after infection with the rotavirus A5-16 strain. In cells either infected with A5-13 or transfected with pcD-NSP1, coimmunoprecipitation of NSP1 with phosphoinositide 3-kinase (PI3K) was observed, indicating that strong activation of PI3K\/Akt could be due to its interaction with NSP1. In addition, after infection with same multiplicity of infection, A5-16 showed reduced number of viral particles compared to the A5-13 strain at the end of the replication cycle. A lower growth rate could be due to weak induction of PI3K\/Akt and NF-\u03baB, since the A5-13 strain also showed reduced growth in the presence of PI3K or NF-\u03baB inhibitors. This effect was interferon independent; however, it was partly due to significantly higher caspase-3 activity, poly-ADP ribose polymerase (PARP) cleavage, and apoptosis during earlier stages of infection with the NSP1 mutant. Thus, our data suggest that NSP1 positively supports rotavirus growth by suppression of premature apoptosis for improved virus growth after infection.<\/jats:p>","DOI":"10.1128\/jvi.00225-10","type":"journal-article","created":{"date-parts":[[2010,4,15]],"date-time":"2010-04-15T01:34:58Z","timestamp":1271295298000},"page":"6834-6845","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["Rotavirus Nonstructural Protein 1 Suppresses Virus-Induced Cellular Apoptosis To Facilitate Viral Growth by Activating the Cell Survival Pathways during Early Stages of Infection"],"prefix":"10.1128","volume":"84","author":[{"given":"Parikshit","family":"Bagchi","sequence":"first","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipanjan","family":"Dutta","sequence":"additional","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiladitya","family":"Chattopadhyay","sequence":"additional","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anupam","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umesh Chandra","family":"Halder","sequence":"additional","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sagartirtha","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Department of Zoology, University of Calcutta, Ballygunge Circular Road, Kolkata 700019, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobumichi","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Hygeine, Sapporo Medical University, S-1 W-17, Chuo-ku, Sapporo 060-8556, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Komoto","sequence":"additional","affiliation":[{"name":"Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koki","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamta","family":"Chawla-Sarkar","sequence":"additional","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425801"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02020-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408376102"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02498-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.14.7945-7956.2003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01420-06"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040121"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.10.028"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023668705040"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401416"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00232-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19771-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.22.2905"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.4.1865-1872.2004"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.06.044"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01309.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02082-06"},{"key":"e_1_3_2_19_2","first-page":"123","volume":"27","year":"1998","unstructured":"Einerhand, A. W. 1998. Rotavirus NSP4 acts as a viral enterotoxin to induce diarrhea and is a potential target for rotavirus vaccines. J. Pediatr. Gastroenterol. Nutr.27:123-124.","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.4049\/jimmunol.163.3.1457","volume":"163","year":"1999","unstructured":"Elewaut, D., J. A. DiDonato, J. M. Kim, F. Truong, L. Eckmann, and M. F. Kagnoff. 1999. NF-\u03baB is a central regulator of the intestinal epithelial cell innate immune response induced by infection with enteroinvasive bacteria. J. Immunol.163:1457-1466.","journal-title":"J. Immunol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00964.x"},{"key":"e_1_3_2_22_2","first-page":"1917","year":"2007","unstructured":"Estes, M. K., and A. Z. Kapikian. 2007. Rotavirus, p. 1917-1974. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields virology, 5th ed. Lippincott-Raven Publishers, Philadelphia, PA.","journal-title":"Fields virology"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00585-09"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1082604"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000280"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01980-07"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606109103"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00872-08"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.8.677"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1089\/107999099314360"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01450-08"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1492"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2003.12.007"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0470"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.10.010"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1303"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.14.6288"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100499200"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.6.3821-3829.1994"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.13.8388-8399.2005"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.12.7836"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/43466"},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"4442","DOI":"10.4049\/jimmunol.163.8.4442","volume":"163","year":"1999","unstructured":"Rollo, E. E., K. P. Kumar, N. C. Reich, J. Cohen, J. Angel, H. B. Greenberg, R. Sheth, J. Anderson, B. Oh, S. J. Hempson, E. R. Mackow, and R. D. Shaw. 1999. The epithelial cell response to rotavirus infection. J. Immunol.163:4442-4452.","journal-title":"J. Immunol."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.18.9721-9730.2004"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.53.1.577"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01186-09"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82483-0"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-43-3-513"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00245-09"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90404-E"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.8.4363-4369.2004"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312245200"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.17.7989-7996.2000"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.6.4125-4130.1996"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108107200"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00225-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00225-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T21:06:49Z","timestamp":1711400809000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00225-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7]]},"references-count":54,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2010,7]]}},"alternative-id":["10.1128\/JVI.00225-10"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00225-10","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7]]},"assertion":[{"value":"2010-02-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-03-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}