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Mutation of the site in the full-length K-bZIP promoter reduced Rta-mediated transactivation by greater than 80%. The K-bZIP element contains an octamer (Oct) binding site that overlaps the Rta site and is well conserved with Oct elements found in the immediate-early promoters of herpes simplex virus type 1(HSV-1). The cellular protein Oct-1, but not Oct-2, binds to the K-bZIP element in a sequence-specific fashion in vitro and in vivo and stimulates Rta binding to the promoter DNA. The coexpression of Oct-1 enhances Rta-mediated transactivation of the wild type but not the mutant K-bZIP promoter, and Oct-1 and Rta proteins bind to each other directly in vitro. Mutations of Rta within an amino acid sequence conserved with HSV-1 virion protein 16 eliminate Rta's interactions with Oct-1 and K-bZIP promoter DNA but not RBP-Jk. The binding of Rta to both Oct-1 and DNA contributes to the transactivation of the K-bZIP promoter and viral reactivation, and Rta mutants deficient for both interactions are completely debilitated. Our data suggest that the Rta\/Oct-1 interaction is essential for optimal KSHV reactivation. Transfections of mouse embryo fibroblasts and an endothelial cell line suggest cell-specific differences in the requirement for Oct-1 or RBP-Jk in Rta-mediated transactivation of the K-bZIP promoter. We propose a model in which Rta transactivation of the promoter is specified by the combination of DNA binding and interactions with several cellular DNA binding proteins including Oct-1.<\/jats:p>","DOI":"10.1128\/jvi.00265-07","type":"journal-article","created":{"date-parts":[[2007,5,31]],"date-time":"2007-05-31T00:39:15Z","timestamp":1180571955000},"page":"8451-8467","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Direct Interactions of Kaposi's Sarcoma-Associated Herpesvirus\/Human Herpesvirus 8 ORF50\/Rta Protein with the Cellular Protein Octamer-1 and DNA Are Critical for Specifying Transactivation of a Delayed-Early Promoter and Stimulating Viral Reactivation"],"prefix":"10.1128","volume":"81","author":[{"given":"Kyla Driscoll","family":"Carroll","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey\/New Jersey Medical School, Newark, New Jersey 07103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farah","family":"Khadim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey\/New Jersey Medical School, Newark, New Jersey 07103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia","family":"Spadavecchia","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey\/New Jersey Medical School, Newark, New Jersey 07103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Palmeri","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey\/New Jersey Medical School, Newark, New Jersey 07103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Lukac","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey\/New Jersey Medical School, Newark, New Jersey 07103"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7725108"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.63.6.2798-2812.1989"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.11.1400"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.8.4952-4964.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.3.542"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)10004-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.10.7963-7968.1997"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1295-1274"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.24.12574-12583.2002"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.02.007"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90240-Y"},{"key":"e_1_3_2_13_2","first-page":"5788","volume":"81","year":"2006","unstructured":"Bu, W., K. 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