{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T15:03:08Z","timestamp":1789916588904,"version":"4.0.1"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The New World arenaviruses, Junin, Machupo, Guanarito, Sabia, and Chapare, are associated with rapidly progressing severe hemorrhagic fever with a high rate of case fatality in various regions of South America. The threat of natural or deliberate outbreaks associated with these viruses makes the development of preventive or therapeutic measures important. Here we describe a Junin virus functional minigenome system and a reverse genetics system for production of infectious Junin virus. This robust, highly efficient system involves transfection of cells with only two plasmids which transcribe the virus S and L antigenomic RNAs. The utility of the system is demonstrated by generating Junin viruses which encode a glycoprotein precursor (GPC) containing the following: (i) the wild-type (SKI-1\/S1P peptidase) cleavage site, (ii) no cleavage site, or (iii) a cleavage site where the SKI-1\/S1P motif (RSLK) is replaced by a furin cleavage site (RRKR). In contrast to the wild-type virus, Junin virus lacking a GPC cleavage site replicated within successfully transfected cells but failed to yield infectious virus particles. This confirms observations with other arenaviruses suggesting that GPC cleavage is essential for arenavirus infectivity. In contrast, infectious Junin virus which encoded GPC cleaved by furin-like proteases was easily generated. The two-plasmid, high efficiency aspects of this Junin virus reverse genetics system show great promise for addressing important questions regarding arenavirus hemorrhagic fever disease and for development of precisely attenuated live arenavirus vaccines.<\/jats:p>","DOI":"10.1128\/jvi.00276-09","type":"journal-article","created":{"date-parts":[[2009,3,26]],"date-time":"2009-03-26T01:09:42Z","timestamp":1238029782000},"page":"5606-5614","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["Efficient Reverse Genetics Generation of Infectious Junin Viruses Differing in Glycoprotein Processing"],"prefix":"10.1128","volume":"83","author":[{"given":"Ce\u0301sar G.","family":"Albarin\u0303o","sequence":"first","affiliation":[{"name":"Special Pathogens Branch"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E\u0301ric","family":"Bergeron","sequence":"additional","affiliation":[{"name":"Special Pathogens Branch"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bobbie Rae","family":"Erickson","sequence":"additional","affiliation":[{"name":"Special Pathogens Branch"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina L.","family":"Khristova","sequence":"additional","affiliation":[{"name":"Biotechnology Core Facility Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G-14, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre E.","family":"Rollin","sequence":"additional","affiliation":[{"name":"Special Pathogens Branch"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart T.","family":"Nichol","sequence":"additional","affiliation":[{"name":"Special Pathogens Branch"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02778-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-78-7-1605"},{"key":"e_1_3_2_4_2","first-page":"118","volume":"25","year":"1991","unstructured":"Barrera Oro, J. G., and K. T. McKee, Jr. 1991. Toward a vaccine against Argentine hemorrhagic fever. Bull. Pan Am. Health Organ.25:118-126.","journal-title":"Bull. Pan Am. Health Organ."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01647-07"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020051"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.5.2866-2872.2003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.01.046"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.3.1187-1199.2000"},{"key":"e_1_3_2_10_2","first-page":"1792","volume":"2","year":"2007","unstructured":"Buchmeier, M. J., C. J. Peters, and J. C. de la Torre. 2007. Arenaviridae: the viruses and their replication, p. 1792-1827. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 5th ed., vol. 2. Lippincott, Williams and Wilkins, Philadelphia, PA.","journal-title":"Fields virology"},{"key":"e_1_3_2_11_2","first-page":"41","volume":"37","year":"1993","unstructured":"Contigiani, M., S. Medeot, and G. Diaz. 1993. Heterogeneity and stability characteristics of Candid 1 attenuated strain of Junin virus. Acta Virol.37:41-46.","journal-title":"Acta Virol."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2008.08.002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.13.6107-6114.2001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0600652103"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.12.6196-6203.1990"},{"key":"e_1_3_2_16_2","first-page":"73","volume":"46","year":"1986","unstructured":"Gardenal, C. N., M. S. Sabattini, and A. Blanco. 1986. Geographic patterns of allele frequencies in Calomys musculinus reservoir-host of Junin virus. Medicina (Buenos Aires)46:73-78.","journal-title":"Medicina (Buenos Aires)"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.09.082"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-005-5843-2"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.24.13793-13803.2004"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(03)00421-5"},{"key":"e_1_3_2_21_2","first-page":"175","volume":"262","year":"2002","unstructured":"Lee, K. J., and J. C. de la Torre. 2002. Reverse genetics of arenaviruses. Curr. Top. Microbiol. Immunol.262:175-193.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.8.3470-3477.2000"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221447598"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.24.12241-12251.2001"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.10.009"},{"key":"e_1_3_2_26_2","first-page":"567","volume":"52","year":"1975","unstructured":"Maiztegui, J. I. 1975. Clinical and epidemiological patterns of Argentine haemorrhagic fever. Bull. W.H.O.52:567-575.","journal-title":"Bull. W.H.O."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1086\/514211"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1159\/000150276"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.51.554"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1991.44.589"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01368-06"},{"key":"e_1_3_2_32_2","first-page":"2300","volume":"30","year":"1958","unstructured":"Parodi, A. S., D. J. Greenway, H. R. Rugiero, M. Frigerio, J. M. De La Barrera, N. Mettler, F. Garzon, M. Boxaca, L. Guerrero, and N. Nota. 1958. Concerning the epidemic outbreak in Junin. Dia Med.30:2300-2301. (In Spanish.)","journal-title":"Dia Med."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1332709100"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02392-07"},{"key":"e_1_3_2_35_2","first-page":"221","volume":"78","year":"1964","unstructured":"Rugiero, H. R., A. S. Parodi, H. G. Rugiero, N. E. Mettler, M. Boxaca, A. L. Guerrero, A. Cintora, C. Magnoni, H. Milani, F. Maglio, C. Cambaceres, L. Astarloa, G. Squassi, D. Fernandez, and A. Giacosa. 1964. Fiebre hemorragica Argentina. I. Periodo de incubacion e invasion. Rev. Assoc. Med. Argent.78:221-226.","journal-title":"Rev. Assoc. Med. Argent."},{"key":"e_1_3_2_36_2","first-page":"313","volume":"34","year":"1974","unstructured":"Sabattini, M. S., J. G. Barrera Oro, J. I. Maiztegui, and B. R. de Ferradas. 1974. Activity of lymphocytic choriomeningitis virus in the endemic area of Argentine hemorrhagic fever (AHF) II. Isolation from a field Mus musculus captured in Southeast Cordoba. Medicina (Buenos Aires)34:313-320. (In Spanish.)","journal-title":"Medicina (Buenos Aires)"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-70-5-1125"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.01.012"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm934"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.8.4330-4341.2004"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.16.8640-8649.2003"},{"key":"e_1_3_2_42_2","first-page":"79","volume":"134","year":"1987","unstructured":"Weissenbacher, M. C., R. P. Laguens, and C. E. Coto. 1987. Argentine hemorrhagic fever. Curr. Top. Microbiol. Immunol.134:79-116.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.19.10783-10792.2004"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00276-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00276-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:29:32Z","timestamp":1646483372000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00276-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":42,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1128\/JVI.00276-09"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00276-09","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6]]},"assertion":[{"value":"2009-02-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-03-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}