{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:48:10Z","timestamp":1791114490252,"version":"4.1.0"},"reference-count":71,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2006,8,15]],"date-time":"2006-08-15T00:00:00Z","timestamp":1155600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2006,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The human herpesvirus Epstein-Barr virus (EBV) establishes latency and\npromotes the long-term survival of its host B cell by targeting the\nmolecular machinery controlling cell fate decisions. The cellular\nantiapoptotic\n            <jats:italic>bfl-1<\/jats:italic>\n            gene confers protection from apoptosis\nunder conditions of growth factor deprivation when expressed\nectopically in an EBV-negative Burkitt's lymphoma-derived cell line (B.\nD'Souza, M. Rowe, and D. Walls, J. Virol.\n74:6652-6658, 2000), and the EBV latent membrane protein 1\n(LMP1) and its cellular functional homologue CD40 can both drive\n            <jats:italic>bfl-1<\/jats:italic>\n            via an NF-\u03baB-dependent enhancer element in the\n            <jats:italic>bfl-1<\/jats:italic>\n            promoter (B. N. D'Souza, L. C.\nEdelstein, P. M. Pegman, S. M. Smith, S. \nT. Loughran, A. Clarke, A. Mehl, M. Rowe, C. Ge\u0301linas, and D.\nWalls, J. Virol. 78:1800-1816, 2004). Here we show\nthat the EBV nuclear antigen 2 (EBNA2) also upregulates\n            <jats:italic>bfl-1<\/jats:italic>\n            .\nEBNA2\n            <jats:italic>trans<\/jats:italic>\n            -activation of\n            <jats:italic>bfl-1<\/jats:italic>\n            requires CBF1 (or\nRBP-J\u03ba), a nuclear component of the Notch signaling pathway,\nand there is an essential role for a core consensus CBF1-binding site\non the\n            <jats:italic>bfl-1<\/jats:italic>\n            promoter.\n            <jats:italic>trans<\/jats:italic>\n            -activation is dependent\non the EBNA2-CBF1 interaction, is modulated by other EBV gene products\nknown to interact with the CBF1 corepressor complex, and does not\ninvolve activation of NF-\u03baB.\n            <jats:italic>bfl-1<\/jats:italic>\n            expression is\ninduced and maintained at high levels by the EBV growth program in a\nlymphoblastoid cell line, and withdrawal of either EBNA2 or LMP1 does\nnot lead to a reduction in\n            <jats:italic>bfl-1<\/jats:italic>\n            mRNA levels in this context,\nwhereas the simultaneous loss of both EBV proteins results in a major\ndecrease in\n            <jats:italic>bfl-1<\/jats:italic>\n            expression. These findings are relevant to\nour understanding of EBV persistence, its role in malignant disease,\nand the B-cell developmental\nprocess.\n          <\/jats:p>","DOI":"10.1128\/jvi.00278-06","type":"journal-article","created":{"date-parts":[[2006,7,27]],"date-time":"2006-07-27T01:18:55Z","timestamp":1153963135000},"page":"8133-8144","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Epstein-Barr Virus Nuclear Antigen 2\n            <i>trans<\/i>\n            -Activates the Cellular Antiapoptotic\n            <i>bfl-1<\/i>\n            Gene by a CBF1\/RBPJ\u03ba-Dependent Pathway"],"prefix":"10.1128","volume":"80","author":[{"given":"Pamela M.","family":"Pegman","sequence":"first","affiliation":[{"name":"School of Biotechnology and National Centre for Sensor\rResearch"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sine\u0301ad M.","family":"Smith","sequence":"additional","affiliation":[{"name":"School of Biotechnology and National Centre for Sensor\rResearch"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan N.","family":"D'Souza","sequence":"additional","affiliation":[{"name":"School of Biotechnology and National Centre for Sensor\rResearch"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sine\u0301ad T.","family":"Loughran","sequence":"additional","affiliation":[{"name":"School of Biotechnology and National Centre for Sensor\rResearch"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Maier","sequence":"additional","affiliation":[{"name":"Institut fur Klinische Molekularbiologie und Tumorgenetik, GSF-Forschungszentrum fur Umwelt und Gesundheit, Marchioninistrasse 25, D-81377 Munchen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"Kempkes","sequence":"additional","affiliation":[{"name":"Institut fur Klinische Molekularbiologie und Tumorgenetik, GSF-Forschungszentrum fur Umwelt und Gesundheit, Marchioninistrasse 25, D-81377 Munchen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Cahill","sequence":"additional","affiliation":[{"name":"Vascular Health Research Centre, Faculty of Science\rand Health, Dublin City University, Dublin 9, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Simmons","sequence":"additional","affiliation":[{"name":"Centre for Advanced Biotechnology and Medicine and Department of Biochemistry, UMDNJ-Robert Wood Johnson Medical School,\rPiscataway, New Jersey 08854"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301line","family":"Ge\u0301linas","sequence":"additional","affiliation":[{"name":"Centre for Advanced Biotechnology and Medicine and Department of Biochemistry, UMDNJ-Robert Wood Johnson Medical School,\rPiscataway, New Jersey 08854"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dermot","family":"Walls","sequence":"additional","affiliation":[{"name":"School of Biotechnology and National Centre for Sensor\rResearch"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90893-G"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910470412"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.3.369"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.10.2803"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910190105"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.100119497"},{"key":"e_1_3_2_8_2","first-page":"1693","volume":"11","year":"1995","unstructured":"Choi, S. S., I. C. Park, J. W. Yun, Y. C. Sung, S. I. Hong, and H. S. Shin. 1995. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene11:1693-1698.","journal-title":"Oncogene"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.23.9558"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201851"},{"key":"e_1_3_2_11_2","first-page":"3879","volume":"56","year":"1996","unstructured":"D'Sa-Eipper, C., T. Subramanian, and G. Chinnadurai. 1996. bfl-1, a bcl-2 homologue, suppresses p53-induced apoptosis and exhibits potent cooperative transforming activity. Cancer Res.56:3879-3882.","journal-title":"Cancer Res."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.14.6652-6658.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.4.1800-1816.2004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306482101"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0452"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.12.8653-8659.1996"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.13.5899-5912.2001"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/349612a0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-71-7-1481"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.5.2025"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2004.04.018"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90007-L"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.4.2770-2780.1999"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.3.952"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.1.23"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1040-8428(00)00046-9"},{"key":"e_1_3_2_27_2","first-page":"549","volume":"10","year":"1995","unstructured":"Huen, D. S., S. A. Henderson, D. Croom-Carter, and M. Rowe. 1995. The Epstein-Barr virus latent membrane protein-1 (LMP1) mediates activation of NF-kappa B and cell surface phenotype via two effector regions in its carboxy-terminal cytoplasmic domain. Oncogene10:549-560.","journal-title":"Oncogene"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-74264-7_13"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.2001.0407"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.1.253-262.1995"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.44.27201"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.8.3089.bloodjournal8783089"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)01046-0"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.20.4133"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.16.10709-10717.2005"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.0084"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb06978.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-2-227"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200898"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.6.1700"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203502"},{"key":"e_1_3_2_42_2","first-page":"7985","volume":"61","year":"2001","unstructured":"Kita, D., T. Takino, M. Nakada, T. Takahashi, J. Yamashita, and H. Sato. 2001 . Expression of dominant-negative form of Ets-1 suppresses fibronectin-stimulated cell adhesion and migration through down-regulation of integrin alpha5 expression in U251 glioma cell line.Cancer Res.61:7985-7991.","journal-title":"Cancer Res."},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06900.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.22.12694-12697.2004"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.182552499"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1687"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-9-2117"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.3.1944-1950.1995"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.9.5375-5383.1994"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.20.9237"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.14.8784-8792.2005"},{"key":"e_1_3_2_53_2","first-page":"276","volume":"22","year":"1975","unstructured":"Menezes, J., W. Leibold, G. Klein, and G. Clements. 1975. Establishment and characterization of an Epstein-Barr virus (EBC)-negative lymphoblastoid B cell line (BJA-B) from an exceptional, EBV-genome-negative African Burkitt's lymphoma.Biomedicine22:276-284.","journal-title":"Biomedicine"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.5.2679"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199904000-00003"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211051200"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)01156-3"},{"key":"e_1_3_2_58_2","first-page":"293","volume":"55","year":"1997","unstructured":"Sakai, T., and T. Honjo. 1997. [Transcriptional activity of EBNA2 through RBP-J.]. Nippon Rinsho.55:293-298.","journal-title":"Nippon Rinsho."},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204034"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-76-11-2669"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.7.3082-3092.2000"},{"key":"e_1_3_2_62_2","first-page":"1521","volume":"12","year":"1996","unstructured":"Tornell, J., S. Farzad, A. Espander-Jansson, G. Matejka, O. Isaksson, and L. Rymo. 1996. Expression of Epstein-Barr nuclear antigen 2 in kidney tubule cells induce tumors in transgenic mice.Oncogene12:1521-1528.","journal-title":"Oncogene"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.6.965"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.1.289"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201469200"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1578"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.15.11092"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.6.2946-2956.2001"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.11.5151-5160.2000"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb01098.x"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05642.x"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.2001.0409"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00278-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00278-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:19:29Z","timestamp":1646475569000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00278-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8,15]]},"references-count":71,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2006,8,15]]}},"alternative-id":["10.1128\/JVI.00278-06"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00278-06","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,8,15]]},"assertion":[{"value":"2006-02-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-05-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-08-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}