{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:33:59Z","timestamp":1787016839750,"version":"build-2736575974"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2008,8,15]],"date-time":"2008-08-15T00:00:00Z","timestamp":1218758400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2008,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Feline immunodeficiency virus (FIV) is the\n                    <jats:italic>Lentivirus<\/jats:italic>\n                    responsible for an immunodeficiency-like disease in domestic cats (\n                    <jats:italic>Felis catus<\/jats:italic>\n                    ). FIV is divided into five phylogenetic subtypes (A, B, C, D, and E), based on genetic diversity. Knowledge of the geographical distribution of subtypes is relevant for understanding different disease progressions and for vaccine development. In this study, viral sequences of 26 infected cats from Rio de Janeiro, 8 undergoing treatment with zidovudine (AZT) for at least 5 years, were successfully amplified from blood specimens.\n                    <jats:italic>gag<\/jats:italic>\n                    capsid (CA),\n                    <jats:italic>pol<\/jats:italic>\n                    reverse transcriptase (RT), and\n                    <jats:italic>env<\/jats:italic>\n                    gp120 (V3-V4) regions were analyzed to determine subtypes and to evaluate potential mutations related to antiretroviral drug resistance among treated cats. Subtyping based on phylogenetic analysis was performed by the neighbor-joining and maximum likelihood methods. All of the sequences clustered with subtype B in the three regions, exhibiting low genetic variability. Additionally, we found evidence that the same virus is circulating in animals in close contact. The analysis of FIV RT sequences identified two new putative mutations related to drug resistance located in the RT \u201cfinger\u201d domain, which has 60% identity to human immunodeficiency virus (HIV) sequence. Amino acid change K\u2192R at codons 64 and 69 was found in 25% and 37.5% of the treated animals, respectively. These signatures were comparable to K65R and K70R thymidine-associated mutations found in the HIV-1 HXB2 counterpart. This finding strongly suggests a position correlation between the mutations found in FIV and the K65R and K70R substitutions from drug-resistant HIV-1 strains.\n                  <\/jats:p>","DOI":"10.1128\/jvi.00310-08","type":"journal-article","created":{"date-parts":[[2008,6,12]],"date-time":"2008-06-12T21:04:06Z","timestamp":1213304646000},"page":"7863-7874","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Phylogenetic and Genetic Analysis of Feline Immunodeficiency Virus\n                    <i>gag<\/i>\n                    ,\n                    <i>pol<\/i>\n                    , and\n                    <i>env<\/i>\n                    Genes from Domestic Cats Undergoing Nucleoside Reverse Transcriptase Inhibitor Treatment or Treatment-Nai\u0308ve Cats in Rio de Janeiro, Brazil"],"prefix":"10.1128","volume":"82","author":[{"given":"Angelica N.","family":"Martins","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Virology, Department of Genetics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila O.","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Virology, Department of Genetics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose P.","family":"Simonetti","sequence":"additional","affiliation":[{"name":"Department of Virology, FIOCRUZ, Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann G.","family":"Schatzmayr","sequence":"additional","affiliation":[{"name":"Laboratory of Flavivirus, Department of Virology, FIOCRUZ, Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ami\u0301lcar","family":"Tanuri","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Virology, Department of Genetics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo M.","family":"Brindeiro","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Virology, Department of Genetics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(01)00426-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi981200e"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.6.4241-4253.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/165.1.105"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2000000100002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9402"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-006-3363-8"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01309743"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.2.275"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/315317"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02090-06"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-1-93"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.23.10958-10964.2000"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.2.1023-1028.2000"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050172"},{"key":"e_1_3_2_17_2","first-page":"221","volume":"194","year":"1989","unstructured":"Ishida, T., T. 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