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In the present study, the immunogenicity of recombinant RABV expressing MIP-1\u03b1 (rHEP-MIP1\u03b1) was determined. It was found that intramuscular immunization of BALB\/c mice with rHEP-MIP1\u03b1 resulted in a higher level of expression of MIP-1\u03b1 at the site of inoculation, increased recruitment of dendritic cells (DCs) and mature B cells into the draining lymph nodes and the peripheral blood, and higher virus-neutralizing antibody titers than immunization with the parent rHEP and recombinant RABVs expressing RANTES (CCL5) or IP-10 (CXCL10). Our data thus demonstrate that expression of MIP-1\u03b1 not only reduces viral pathogenicity but also enhances immunogenicity by recruiting DCs and B cells to the site of immunization, the lymph nodes, and the blood.<\/jats:p>","DOI":"10.1128\/jvi.00326-10","type":"journal-article","created":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T02:14:51Z","timestamp":1277950491000},"page":"9642-9648","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Expression of MIP-1\u03b1 (CCL3) by a Recombinant Rabies Virus Enhances Its Immunogenicity by Inducing Innate Immunity and Recruiting Dendritic Cells and B Cells"],"prefix":"10.1128","volume":"84","author":[{"given":"Ling","family":"Zhao","sequence":"first","affiliation":[{"name":"Departments of Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harufusa","family":"Toriumi","sequence":"additional","affiliation":[{"name":"Departments of Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualei","family":"Wang","sequence":"additional","affiliation":[{"name":"Departments of Pathology"},{"name":"College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Kuang","sequence":"additional","affiliation":[{"name":"Departments of Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Guo","sequence":"additional","affiliation":[{"name":"Departments of Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinjiro","family":"Morimoto","sequence":"additional","affiliation":[{"name":"Yasuda Women's University, Hiroshima 731-0153, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen F.","family":"Fu","sequence":"additional","affiliation":[{"name":"Departments of Pathology"},{"name":"Infectious Diseases, University of Georgia, Athens, GA 30602"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.16.8729-8735.2003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/8.3.405"},{"key":"e_1_3_2_4_2","first-page":"1204","volume":"58","year":"2009","unstructured":"Centers for Disease Control and Prevention. 2009. 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