{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:52:45Z","timestamp":1791251565802,"version":"4.1.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2009,7,15]],"date-time":"2009-07-15T00:00:00Z","timestamp":1247616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2009,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Arenaviruses, such as Tacaribe virus (TacV) and its closely related pathogenic Junin virus (JunV), are enveloped viruses with a bipartite negative-sense RNA genome that encodes the nucleocapsid protein (N), the precursor of the envelope glycoprotein complex (GP), the polymerase (L), and a RING finger protein (Z), which is the driving force of arenavirus budding. We have established a plasmid-based system which allowed the successful packaging of TacV-like nucleocapsids along with Z and GP of JunV into infectious virus-like particles (VLPs). By coexpressing different combinations of the system components, followed by biochemical analysis of the VLPs, the requirements for the assembly of both N and GP into particles were defined. We found that coexpression of N with Z protein in the absence of minigenome and other viral proteins was sufficient to recruit N within lipid-enveloped Z-containing VLPs. In addition, whereas GP was not required for the incorporation of N, coexpression of N substantially enhanced the ratio of GP to Z into VLPs. Disruption of the RING structure or mutation of residue L79 to alanine within Z protein, although it had no effect on Z self-budding, severely impaired VLP infectivity. These mutations drastically altered intracellular Z-N interactions and the incorporation of both N and GP into VLPs. Our results support the conclusion that the interaction between Z and N is required for assembly of both the nucleocapsids and the glycoproteins into infectious arenavirus budding particles.<\/jats:p>","DOI":"10.1128\/jvi.00329-09","type":"journal-article","created":{"date-parts":[[2009,5,7]],"date-time":"2009-05-07T00:34:48Z","timestamp":1241656488000},"page":"7029-7039","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["The RING Domain and the L79 Residue of Z Protein Are Involved in both the Rescue of Nucleocapsids and the Incorporation of Glycoproteins into Infectious Chimeric Arenavirus-Like Particles"],"prefix":"10.1128","volume":"83","author":[{"given":"Juan Cruz","family":"Casabona","sequence":"first","affiliation":[{"name":"Centro de Virologi\u0301a Animal (CEVAN), Instituto de Ciencia y Tecnologi\u0301a Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnologi\u0301a (CONICET), Saladillo 2468, C1440FFX Ciudad Auto\u0301noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesica M.","family":"Levingston Macleod","sequence":"additional","affiliation":[{"name":"Centro de Virologi\u0301a Animal (CEVAN), Instituto de Ciencia y Tecnologi\u0301a Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnologi\u0301a (CONICET), Saladillo 2468, C1440FFX Ciudad Auto\u0301noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Eugenia","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Centro de Virologi\u0301a Animal (CEVAN), Instituto de Ciencia y Tecnologi\u0301a Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnologi\u0301a (CONICET), Saladillo 2468, C1440FFX Ciudad Auto\u0301noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo A.","family":"Gomez","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones en Qui\u0301mica Biolo\u0301gica de Co\u0301rdoba (CIQUIBIC, UNC-CONICET), Departamento de Qui\u0301mica Biolo\u0301gica, Facultad de Ciencias Qui\u0301micas, Universidad Nacional de Co\u0301rdoba, Co\u0301rdoba X5000HUA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Lopez","sequence":"additional","affiliation":[{"name":"Centro de Virologi\u0301a Animal (CEVAN), Instituto de Ciencia y Tecnologi\u0301a Dr. Cesar Milstein, Consejo Nacional de Ciencia y Tecnologi\u0301a (CONICET), Saladillo 2468, C1440FFX Ciudad Auto\u0301noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"36","volume":"11","year":"2004","unstructured":"Abramoff, M. 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