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Animals were immunized intramuscularly with a peptide containing one copy of a FMDV T-cell epitope and branching out into four copies of a B-cell epitope. The four immunized pigs did not develop significant clinical signs upon FMDV challenge, neither systemic nor mucosal FMDV replication, nor was its transmission to contact control pigs observed. The dendrimeric construction specifically induced high titers of FMDV-neutralizing antibodies and activated FMDV-specific T cells. Interestingly, a potent anti-FMDV immunoglobulin A response (local and systemic) was observed, despite the parenteral administration of the peptide. On the other hand, peptide-immunized animals showed no antibodies specific of FMDV infection, which qualifies the peptide as a potential marker vaccine. Overall, the dendrimeric peptide used elicited an immune response comparable to that found for control FMDV-infected pigs that correlated with a solid protection against FMDV challenge. Dendrimeric designs of this type may hold substantial promise for peptide subunit vaccine development.<\/jats:p>","DOI":"10.1128\/jvi.00401-08","type":"journal-article","created":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:54:02Z","timestamp":1209603242000},"page":"7223-7230","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":103,"title":["Enhanced Mucosal Immunoglobulin A Response and Solid Protection against Foot-and-Mouth Disease Virus Challenge Induced by a Novel Dendrimeric Peptide"],"prefix":"10.1128","volume":"82","author":[{"given":"Carolina","family":"Cubillos","sequence":"first","affiliation":[{"name":"Centro de Investigacio\u0301n en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz G.","family":"de la Torre","sequence":"additional","affiliation":[{"name":"Departament de Cie\u0300ncies Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annamaria","family":"Jakab","sequence":"additional","affiliation":[{"name":"Departament de Cie\u0300ncies Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giorgia","family":"Clementi","sequence":"additional","affiliation":[{"name":"Departament de Cie\u0300ncies Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Borra\u0301s","sequence":"additional","affiliation":[{"name":"Departament de Cie\u0300ncies Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Ba\u0301rcena","sequence":"additional","affiliation":[{"name":"Centro de Investigacio\u0301n en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Andreu","sequence":"additional","affiliation":[{"name":"Departament de Cie\u0300ncies Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Sobrino","sequence":"additional","affiliation":[{"name":"Centro de Investigacio\u0301n en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain"},{"name":"Centro de Biologi\u0301a Molecular Severo Ochoa (CSIC-UAM), Canto blanco, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"Blanco","sequence":"additional","affiliation":[{"name":"Centro de Investigacio\u0301n en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/337709a0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.07.034"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(96)00055-2"},{"key":"e_1_3_2_5_2","unstructured":"Modern inactivated foot-and-mouth disease (FMD) vaccines: historical background and key elements in production and use 2004"},{"key":"e_1_3_2_6_2","first-page":"145","year":"2004","unstructured":"Baxt, B., and E. 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