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Here we show, for the first time,<jats:italic>de novo<\/jats:italic>formation of ChHV5-positive intranuclear inclusions in cultured green turtle cells, which is indicative of active lytic replication of the virus. The minimal requirements to achieve lytic replication in cultured cells included (i) either<jats:italic>in vitro<\/jats:italic>cultures of ChHV5-positive tumor biopsy specimens (plugs) or organotypic cultures (rafts) consisting of ChHV5-positive turtle fibroblasts in collagen rafts seeded with turtle keratinocytes and (ii) keratinocyte maturation induced by raising raft or biopsy cultures to the air-liquid interface. Virus growth was confirmed by detailed electron microscopic studies that revealed intranuclear sun-shaped capsid factories, tubules, various stages of capsid formation, nuclear export by budding into the perinuclear space, tegument formation, and envelopment to complete<jats:italic>de novo<\/jats:italic>virus production. Membrane synthesis was also observed as a sign of active viral replication. Interestingly, cytoplasmic particles became associated with keratin filaments, a feature not seen in conventional monolayer cell cultures, in which most studies of herpesvirus replication have been performed. Our findings draw a rich and realistic picture of ChHV5 replication in cells derived from its natural host and may be crucial not only to better understand ChHV5 circulation but also to eventually complete Koch's postulates for FP. Moreover, the principles described here may serve as a model for culture of other viruses that are resistant to replication in conventional cell culture.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>A major challenge in virology is the study of viruses that cannot be grown in the laboratory. One example is chelonid herpesvirus 5 (ChHV5), which is associated with fibropapillomatosis, a globally distributed, debilitating, and fatal tumor disease of endangered marine turtles. Pathological examination shows that ChHV5 is shed in skin. Here we show that ChHV5 will grow<jats:italic>in vitro<\/jats:italic>if we replicate the complex three-dimensional structure of turtle skin. Moreover, lytic virus growth requires a close interplay between fibroblasts and keratinocytes. Finally, the morphogenesis of herpesviral growth in three-dimensional cultures reveals a far richer, and likely more realistic, array of capsid morphologies than that encountered in traditional monolayer cell cultures. Our findings have applications to other viruses, including those of humans.<\/jats:p>","DOI":"10.1128\/jvi.00404-17","type":"journal-article","created":{"date-parts":[[2017,6,15]],"date-time":"2017-06-15T00:40:24Z","timestamp":1497487224000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["<i>In Vitro<\/i>Replication of Chelonid Herpesvirus 5 in Organotypic Skin Cultures from Hawaiian Green Turtles (Chelonia mydas)"],"prefix":"10.1128","volume":"91","author":[{"given":"Thierry M.","family":"Work","sequence":"first","affiliation":[{"name":"U.S. Geological Survey, National Wildlife Health Center, Honolulu Field Station, Honolulu, Hawaii, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Dagenais","sequence":"additional","affiliation":[{"name":"U.S. Geological Survey, National Wildlife Health Center, Honolulu Field Station, Honolulu, Hawaii, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina M.","family":"Weatherby","sequence":"additional","affiliation":[{"name":"Biological EM Facility, University of Hawaii, Honolulu, Hawaii, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George H.","family":"Balazs","sequence":"additional","affiliation":[{"name":"NOAA, National Marine Fisheries Service, Pacific Islands Fisheries Science Center, Honolulu, Hawaii, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Ackermann","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1","volume-title":"The biology of sea turtles","author":"Pritchard PCH","year":"1996","unstructured":"Pritchard PCH . 1996. 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