{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T04:23:34Z","timestamp":1771993414743,"version":"3.50.1"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,6,15]],"date-time":"2013-06-15T00:00:00Z","timestamp":1371254400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus's restricted host and cell type tropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251 monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize VZV protein expression\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in situ<\/jats:italic>\n            . Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indirect immunofluorescence (IF); 13 were detected by WB only, and 2 were detected by IF only. A large proportion of viral proteins was analyzed for the first time in the context of virus infection. Our study revealed the subcellular localization of 46 proteins, 14 of which were analyzed in detail by confocal microscopy. Seven viral proteins were analyzed in time course experiments and showed a cascade-like temporal gene expression pattern similar to those of other herpesviruses. Furthermore, selected MAbs tested positive on human skin lesions by using immunohistochemistry, demonstrating the wide applicability of the MAb collection. Finally, a significant portion of the VZV-specific antibodies reacted with orthologs of simian varicella virus (SVV), thus enabling the systematic analysis of varicella in a nonhuman primate model system. In summary, this study provides insight into the potential function of numerous VZV proteins and novel tools to systematically study VZV and SVV pathogenesis.\n          <\/jats:p>","DOI":"10.1128\/jvi.00407-13","type":"journal-article","created":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T00:17:19Z","timestamp":1366244239000},"page":"6943-6954","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Comprehensive Analysis of Varicella-Zoster Virus Proteins Using a New Monoclonal Antibody Collection"],"prefix":"10.1128","volume":"87","author":[{"given":"Tihana","family":"Lenac Rovi\u0161","sequence":"first","affiliation":[{"name":"Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne M.","family":"Bailer","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institut, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, M\u00fcnchen, Germany"},{"name":"University of Stuttgart, Biological Interfacial Engineering, Stuttgart, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkata R.","family":"Pothineni","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institut, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, M\u00fcnchen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner J. D.","family":"Ouwendijk","sequence":"additional","affiliation":[{"name":"Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hrvoje","family":"\u0160imi\u0107","sequence":"additional","affiliation":[{"name":"Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Babi\u0107","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karmela","family":"Mikli\u0107","sequence":"additional","affiliation":[{"name":"Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzana","family":"Mali\u0107","sequence":"additional","affiliation":[{"name":"Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marieke C.","family":"Verweij","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armin","family":"Baiker","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institut, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, M\u00fcnchen, Germany"},{"name":"Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orland","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Institute for Bioinformatics, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, M\u00fcnchen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albrecht","family":"von Brunn","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institut, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, M\u00fcnchen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Zimmer","sequence":"additional","affiliation":[{"name":"Institute for Bioinformatics, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, M\u00fcnchen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Fr\u00fch","sequence":"additional","affiliation":[{"name":"Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georges M. 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