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Furthermore, IAV HA-specific pSIgA antibodies are thought to contribute to cross-protective immunity against multiple IAV subtypes. However, the mechanisms by which pSIgA exerts such versatile antiviral activity are not fully understood. In this study, we generated broadly cross-reactive recombinant IgG and pSIgA having the same antigen-recognition site and compared their antiviral activities\n                    <jats:italic>in vitro<\/jats:italic>\n                    . These recombinant antibodies did not show \u201cclassical\u201d neutralizing activity, whereas pSIgA, but not IgG, significantly inhibited the production of progeny virus particles from infected cells. Plaque formation was also significantly reduced by pSIgA, but not IgG. These effects were seen in infection with IAVs of several different HA subtypes. Based on our findings, we propose an antibody-mediated host defense mechanism by which mucosal immunity may contribute to broad cross-protection from IAVs of multiple HA subtypes, including viruses with pandemic potential.\n                  <\/jats:p>","DOI":"10.1128\/jvi.00408-20","type":"journal-article","created":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T10:02:42Z","timestamp":1585908162000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Potential Role of Nonneutralizing IgA Antibodies in Cross-Protective Immunity against Influenza A Viruses of Multiple Hemagglutinin Subtypes"],"prefix":"10.1128","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7987-9884","authenticated-orcid":false,"given":"Kosuke","family":"Okuya","sequence":"first","affiliation":[{"name":"Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, 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