{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T21:15:00Z","timestamp":1775942100509,"version":"3.50.1"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Activation of CD4\n            <jats:sup>+<\/jats:sup>\n            T cells helps establish and sustain other immune responses. We have previously shown that responses against a broad set of nine CD4\n            <jats:sup>+<\/jats:sup>\n            T-cell epitopes were present in the setting of lymphocytic choriomeningitis virus (LCMV) Armstrong infection in the context of H-2\n            <jats:sup>d<\/jats:sup>\n            . This is quite disparate to the H-2\n            <jats:sup>b<\/jats:sup>\n            setting, where only two epitopes have been identified. We were interested in determining whether a broad set of responses was unique to H-2\n            <jats:sup>d<\/jats:sup>\n            or whether additional CD4\n            <jats:sup>+<\/jats:sup>\n            T-cell epitopes could be identified in the setting of the H-2\n            <jats:sup>b<\/jats:sup>\n            background. To pursue this question, we infected C57BL\/6 mice with LCMV Armstrong and determined the repertoire of CD4\n            <jats:sup>+<\/jats:sup>\n            T-cell responses using overlapping 15-mer peptides corresponding to the LCMV Armstrong sequence. We confirmed positive responses by intracellular cytokine staining and major histocompatibility complex (MHC)-peptide binding assays. A broad repertoire of responses was identified, consisting of six epitopes. These epitopes originate from the nucleoprotein (NP) and glycoprotein (GP). Out of the six newly identified CD4\n            <jats:sup>+<\/jats:sup>\n            epitopes, four of them also stimulate CD8\n            <jats:sup>+<\/jats:sup>\n            T cells in a statistically significant manner. Furthermore, we assessed these CD4\n            <jats:sup>+<\/jats:sup>\n            T-cell responses during the memory phase of LCMV Armstrong infection and after infection with a chronic strain of LCMV and determined that a subset of the responses could be detected under these different conditions. This is the first example of a broad repertoire of shared epitopes between CD4\n            <jats:sup>+<\/jats:sup>\n            and CD8\n            <jats:sup>+<\/jats:sup>\n            T cells in the context of viral infection. These findings demonstrate that immunodominance is a complex phenomenon in the context of helper responses.\n          <\/jats:p>","DOI":"10.1128\/jvi.00435-08","type":"journal-article","created":{"date-parts":[[2008,10,2]],"date-time":"2008-10-02T01:06:41Z","timestamp":1222909601000},"page":"11734-11741","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Lymphocytic Choriomeningitis Virus Infection Yields Overlapping CD4\n            <sup>+<\/sup>\n            and CD8\n            <sup>+<\/sup>\n            T-Cell Responses"],"prefix":"10.1128","volume":"82","author":[{"given":"Courtney","family":"Dow","sequence":"first","affiliation":[{"name":"The La Jolla Institute for Allergy and Immunology, La Jolla, California 92037"},{"name":"Department of Biological Sciences, California State University, San Marcos, California 92096"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Oseroff","sequence":"additional","affiliation":[{"name":"The La Jolla Institute for Allergy and Immunology, La Jolla, California 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bjoern","family":"Peters","sequence":"additional","affiliation":[{"name":"The La Jolla Institute for Allergy and Immunology, La Jolla, California 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Courtney","family":"Nance-Sotelo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, California State University, San Marcos, California 92096"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Sidney","sequence":"additional","affiliation":[{"name":"The La Jolla Institute for Allergy and Immunology, La Jolla, California 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Buchmeier","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute, La Jolla, California 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"The La Jolla Institute for Allergy and Immunology, La Jolla, California 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca R.","family":"Mothe\u0301","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, California State University, San Marcos, California 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