{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T01:02:49Z","timestamp":1771635769498,"version":"3.50.1"},"reference-count":81,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Zoonoses Anticipation and Preparedness Initiative","award":["Grant Agreement No 115760"],"award-info":[{"award-number":["Grant Agreement No 115760"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:p>\n            The proteins N\n            <jats:sup>PRO<\/jats:sup>\n            and E\n            <jats:sup>RNS<\/jats:sup>\n            are unique for the genus\n            <jats:italic>Pestivirus<\/jats:italic>\n            , but only N\n            <jats:sup>PRO<\/jats:sup>\n            has been demonstrated to be nonessential for\n            <jats:italic>in vitro<\/jats:italic>\n            growth. While this was also speculated for E\n            <jats:sup>RNS<\/jats:sup>\n            , it has always been previously shown that pestivirus replicons with deletions of the structural proteins E\n            <jats:sup>RNS<\/jats:sup>\n            , E1, or E2 did not produce any infectious progeny virus in susceptible host cells. Here, we demonstrated for the first time that BuPV E\n            <jats:sup>RNS<\/jats:sup>\n            is dispensable for the generation of infectious virus particles but still important for efficient passaging. The E\n            <jats:sup>RNS<\/jats:sup>\n            -defective BuPV particles showed clearly limited growth in cell culture but were capable of several rounds of infection, expression of foreign genes, and highly efficient\n            <jats:italic>trans<\/jats:italic>\n            -complementation to rescue virus replicon particles (VRPs). The noncytopathic characteristics and the absence of preexisting immunity to BuPV in human populations and livestock also provide a significant benefit for a possible use, e.g., as a vector vaccine platform.\n          <\/jats:p>","DOI":"10.1128\/jvi.00436-20","type":"journal-article","created":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T13:57:52Z","timestamp":1589205472000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Autonomously Replicating RNAs of Bungowannah Pestivirus: E\n            <sup>RNS<\/sup>\n            Is Not Essential for the Generation of Infectious Particles"],"prefix":"10.1128","volume":"94","author":[{"given":"Anja","family":"Dalmann","sequence":"first","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich Loeffler Institute, Greifswald-Insel Riems, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilona","family":"Reimann","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich Loeffler Institute, Greifswald-Insel Riems, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"Wernike","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich Loeffler Institute, Greifswald-Insel Riems, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Beer","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich Loeffler Institute, Greifswald-Insel Riems, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000672"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.11.026"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2007.05.002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1466252315000031"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12035"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2307.170163"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2019.1664940"},{"key":"e_1_3_2_9_2","first-page":"1561","article-title":"Bovine viral diarrhea virus-host cell interactions: adaptation and growth of virus in cell lines","volume":"30","author":"Fernelius AL","year":"1969","unstructured":"Fernelius AL, Lambert G, Hemness GJ. 1969. Bovine viral diarrhea virus-host cell interactions: adaptation and growth of virus in cell lines. Am J Vet Res 30:1561\u20131572.","journal-title":"Am J Vet Res"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.065995-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(88)90673-3"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-69-10-2637"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0749-0720(15)30459-x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.6.5165-5173.1998"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000251"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90625-9"},{"key":"e_1_3_2_17_2","volume-title":"Animal viruses: molecular biology","author":"R\u00fcmenapf T","year":"2008","unstructured":"R\u00fcmenapf T, Thiel H-J. 2008. Molecular biology of pestiviruses. In Mettenleiter TC, Sobrino F (ed), Animal viruses: molecular biology. Caister Academic Press, Norfolk, United Kingdom."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.5.3638-3648.1999"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.74.13.5825-5835.2000"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.76.10.5024-5033.2002"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.9741"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.67.6.3288-3294.1993"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0065-3527(03)59002-9"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.65.9.4705-4712.1991"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-11-2729"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.3.2364-2372.1998"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00699-10"},{"key":"e_1_3_2_28_2","first-page":"845","article-title":"Morphology of bovine viral diarrhea virus","volume":"45","author":"Chu HJ","year":"1984","unstructured":"Chu HJ, Zee YC. 1984. Morphology of bovine viral diarrhea virus. Am J Vet Res 45:845\u2013850.","journal-title":"Am J Vet Res"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-5-1157"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.012559-0"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.08.006"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.9.7681-7684.1998"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-81-2-451"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00672-14"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.18.11901-11913.2005"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M706803200"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.78.10.5507-5512.2004"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8356450"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.70.1.352-358.1996"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.12.10224-10235.1999"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.70.12.8606-8613.1996"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.11.9422-9432.1999"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.74.20.9498-9506.2000"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0042-6822(02)00129-0"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.9.7787-7794.1999"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002598"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.23.12901-12906.2003"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.10.046"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2007.06.007"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.12.007"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2016.09.013"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.05.006"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.01.076"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.6.2.135"},{"key":"e_1_3_2_55_2","first-page":"555","article-title":"Replicon-based vectors of positive strand RNA viruses","volume":"2","author":"Khromykh AA","year":"2000","unstructured":"Khromykh AA. 2000. Replicon-based vectors of positive strand RNA viruses. Curr Opin Mol Ther 2:555\u2013569.","journal-title":"Curr Opin Mol Ther"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2012.01.007"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006028"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1218"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.78.1.136-145.2004"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2008.10.015"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000377"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-70-11-2865"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1080\/14728222.2017.1271415"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.6.3231-3242.2005"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.03.001"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.052688-0"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.09.023"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.74.7.2973-2980.2000"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003973"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.22.12989"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9564"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.9.1495"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines4040039"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2922607"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.7.5967-5977.1998"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02598-15"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.04.001"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00027-18"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.2144\/01311st05"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2011.07.014"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.01.028"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2017.02.011"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00436-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00436-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T14:57:14Z","timestamp":1646492234000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00436-20"}},"subtitle":[],"editor":[{"given":"Mark T.","family":"Heise","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,7]]},"references-count":81,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["10.1128\/JVI.00436-20"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00436-20","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7]]},"assertion":[{"value":"2020-03-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00436-20"}}