{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:07:11Z","timestamp":1790536031307,"version":"4.1.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2009,7,15]],"date-time":"2009-07-15T00:00:00Z","timestamp":1247616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2009,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The coronavirus nucleocapsid protein (N), together with the large, positive-strand RNA viral genome, forms a helically symmetric nucleocapsid. This ribonucleoprotein structure becomes packaged into virions through association with the carboxy-terminal endodomain of the membrane protein (M), which is the principal constituent of the virion envelope. Previous work with the prototype coronavirus mouse hepatitis virus (MHV) has shown that a major determinant of the N-M interaction maps to the carboxy-terminal domain 3 of the N protein. To explore other domain interactions of the MHV N protein, we expressed a series of segments of the MHV N protein as fusions with green fluorescent protein (GFP) during the course of viral infection. We found that two of these GFP-N-domain fusion proteins were selectively packaged into virions as the result of tight binding to the N protein in the viral nucleocapsid, in a manner that did not involve association with either M protein or RNA. The nature of each type of binding was further explored through genetic analysis. Our results defined two strongly interacting regions of the N protein. One is the same domain 3 that is critical for M protein recognition during assembly. The other is domain N1b, which corresponds to the N-terminal domain that has been structurally characterized in detail for two other coronaviruses, infectious bronchitis virus and the severe acute respiratory syndrome coronavirus.<\/jats:p>","DOI":"10.1128\/jvi.00440-09","type":"journal-article","created":{"date-parts":[[2009,5,7]],"date-time":"2009-05-07T00:34:48Z","timestamp":1241656488000},"page":"7221-7234","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":141,"title":["Identification of In Vivo-Interacting Domains of the Murine Coronavirus Nucleocapsid Protein"],"prefix":"10.1128","volume":"83","author":[{"given":"Kelley R.","family":"Hurst","sequence":"first","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York 12201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheri A.","family":"Koetzner","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York 12201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul S.","family":"Masters","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York 12201"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805270106"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.62.11.4280-4287.1988"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.48.31901"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01906-07"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80975-0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02001-08"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11373-005-9035-9"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.02.069"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.2.1164-1179.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02231-06"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.1.580-583.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/13550280260422686"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1412"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(05)64006-7"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.24.12491-12502.2002"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2005.08.021"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.7.5148-5160.1997"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.6.3522-3530.1992"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.2.669-682.2004"},{"key":"e_1_3_2_21_2","first-page":"217","year":"1991","unstructured":"Horton, R. 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