{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:36:47Z","timestamp":1789105007400,"version":"build-2803163510"},"reference-count":113,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T00:00:00Z","timestamp":1658880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T00:00:00Z","timestamp":1658880000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000268","name":"UKRI | Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/P013740\/1"],"award-info":[{"award-number":["BB\/P013740\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"UKRI | Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/P013740\/1"],"award-info":[{"award-number":["BB\/P013740\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["211222_Z_18_Z"],"award-info":[{"award-number":["211222_Z_18_Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["211138\/Z\/18\/Z"],"award-info":[{"award-number":["211138\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2022,7,27]]},"abstract":"<jats:p>We explored the use of rotaviruses (RVs) to express heterologous peptides, using SARS-CoV-2 as an example. Small SARS-CoV-2 peptide insertions (&lt;34 amino acids) into the hypervariable region of the viral protein 4 (VP4) of RV SA11 strain resulted in reduced viral titer and replication, demonstrating a limited tolerance for peptide insertions at this site.<\/jats:p>","DOI":"10.1128\/jvi.00488-22","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T13:01:20Z","timestamp":1656334880000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes"],"prefix":"10.1128","volume":"96","author":[{"given":"Ola","family":"Diebold","sequence":"first","affiliation":[{"name":"Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Venditti","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Sharp","sequence":"additional","affiliation":[{"name":"Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary A.","family":"Blake","sequence":"additional","affiliation":[{"name":"Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfang S.","family":"Tan","sequence":"additional","affiliation":[{"name":"Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Stevens","sequence":"additional","affiliation":[{"name":"Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Caddy","sequence":"additional","affiliation":[{"name":"Cambridge Institute of Therapeutic Immunology and Infectious Disease, Department of Medicine, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0872-9440","authenticated-orcid":true,"given":"Paul","family":"Digard","sequence":"additional","affiliation":[{"name":"Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Borodavka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4473-6373","authenticated-orcid":true,"given":"Eleanor","family":"Gaunt","sequence":"additional","affiliation":[{"name":"Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ1013"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamapediatrics.2018.1960"},{"key":"e_1_3_2_4_2","first-page":"1347","volume-title":"Fields virology.","author":"Estes MK","year":"2013","unstructured":"Estes MK, G HB. 2013. Rotaviruses, p 1347\u20131401. In Knipe DM (ed), Fields virology. Wolters Kluwer Health\/Lippincott Williams & Wilkins: Philadelphia, PA."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.03.020"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2017-14019"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2013.10.019"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.05.017"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD008521.pub6"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.09.107"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix186"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2014.11.007"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-022-03154-2"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.Supplement_1.S98"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-animal-022114-111038"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2007.06.011"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90495-W"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.83"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.793841"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02836"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.13.6052-6061.2001"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.9.5832-5839.1996"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.39.3.879-888.1981"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10996"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.658029"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90888-I"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.11.7940-7947.1996"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2673"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2008.09.006"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2018.08.001"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10236-7"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838200992264"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.7.5411-5421.1999"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.19.5811"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00872-08"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00361-13"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1477"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00437-06"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509385103"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1011948107"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00547-10"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02730-15"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1618424114"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00588-18"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01294-20"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01616-19"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01374-20"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13030363"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-021-05142-7"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aam5434"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI128382"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00959-20"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines9050449"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2798-3"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-021-00292-w"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.08.026"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-020-00243-x"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2814-7"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03324-6"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2599-8"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00402-5"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.09.037"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2180-5"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.322"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-03124-4"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004157"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02460-2"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.27453"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2010.12.002"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2100198118"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-018-2924-9"},{"key":"e_1_3_2_72_2","first-page":"373","article-title":"[In vitro culture of a rotavirus associated with neonatal calf diarrhea]","volume":"7","author":"L\u2019Haridon R","year":"1976","unstructured":"L\u2019Haridon R, Scherrer R. 1976. [In vitro culture of a rotavirus associated with neonatal calf diarrhea] Ann Rech Vet 7:373\u2013381.","journal-title":"Ann Rech Vet"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13071349"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.07.078"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-01549-z"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13010072"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01835-10"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2015.1054583"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.03.011"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00601-08"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1456"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01682-09"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.16.7339-7350.2001"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02461-09"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01228-09"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01346-06"},{"key":"e_1_3_2_87_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008732"},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa052664"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1692"},{"key":"e_1_3_2_90_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(97)00210-7"},{"key":"e_1_3_2_91_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)12106-2"},{"key":"e_1_3_2_92_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145998"},{"key":"e_1_3_2_93_2","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2012.1.1.35"},{"key":"e_1_3_2_94_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2019.197822"},{"key":"e_1_3_2_95_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.1.251-259.1999"},{"key":"e_1_3_2_96_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1035"},{"key":"e_1_3_2_97_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074328"},{"key":"e_1_3_2_98_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.5.885"},{"key":"e_1_3_2_99_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1815591"},{"key":"e_1_3_2_100_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-99642-w"},{"key":"e_1_3_2_101_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100637"},{"key":"e_1_3_2_102_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mec.2021.e00166"},{"key":"e_1_3_2_103_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-5-50"},{"key":"e_1_3_2_104_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc15c03s15"},{"key":"e_1_3_2_105_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-3-63"},{"key":"e_1_3_2_106_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm234"},{"key":"e_1_3_2_107_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_3_2_108_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-2-17"},{"key":"e_1_3_2_109_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.15.10003-10012.2005"},{"key":"e_1_3_2_110_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2004.02.028"},{"key":"e_1_3_2_111_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01634-08"},{"key":"e_1_3_2_112_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00826-09"},{"key":"e_1_3_2_113_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-018-0547-5"},{"key":"e_1_3_2_114_2","doi-asserted-by":"crossref","unstructured":"Blake R Jensen K Mabbott N Hope J Stevens J. 2022. Development of 3D bovine intestinal organoid derived models to investigate Mycobacterium avium ssp paratuberculosis pathogenesis. bioRxiv. https:\/\/www.biorxiv.org\/content\/10.1101\/2022.05.13.491821v1.","DOI":"10.1101\/2022.05.13.491821"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.00488-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.00488-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T19:08:49Z","timestamp":1675969729000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.00488-22"}},"subtitle":[],"editor":[{"given":"Tom","family":"Gallagher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,7,27]]},"references-count":113,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2022,7,27]]}},"alternative-id":["10.1128\/jvi.00488-22"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00488-22","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,27]]},"assertion":[{"value":"2022-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00488-22"}}