{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:52:17Z","timestamp":1791251537263,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Generation of infectious arenavirus-like particles requires the virus RING finger Z protein and surface glycoprotein precursor (GPC) and the correct processing of GPC into GP1, GP2, and a stable signal peptide (SSP). Z is the driving force of arenavirus budding, whereas the GP complex (GPc), consisting of hetero-oligomers of SSP, GP1, and GP2, forms the viral envelope spikes that mediate receptor recognition and cell entry. Based on the roles played by Z and GP in the arenavirus life cycle, we hypothesized that Z and the GPc should interact in a manner required for virion formation. Here, using confocal microscopy and coimmunoprecipitation assays, we provide evidence for subcellular colocalization and biochemical interaction, respectively, of Z and the GPc. Our results from mutation-function analysis reveal that Z myristoylation, but not the Z late (L) or RING domain, is required for Z-GPc interaction. Moreover, Z interacted directly with SSP in the absence of other components of the GPc. We obtained similar results with Z and GPC from the prototypical arenavirus lymphocytic choriomeningitis virus and the hemorrhagic fever arenavirus Lassa fever virus.<\/jats:p>","DOI":"10.1128\/jvi.00499-07","type":"journal-article","created":{"date-parts":[[2007,6,21]],"date-time":"2007-06-21T01:50:37Z","timestamp":1182390637000},"page":"9451-9460","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":94,"title":["Arenavirus Z-Glycoprotein Association Requires Z Myristoylation but Not Functional RING or Late Domains"],"prefix":"10.1128","volume":"81","author":[{"given":"Althea A.","family":"Capul","sequence":"first","affiliation":[{"name":"Molecular and Integrative Neuroscience Department (MIND), Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar","family":"Perez","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neuroscience Department (MIND), Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Burke","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neuroscience Department (MIND), Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Kunz","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neuroscience Department (MIND), Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Buchmeier","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neuroscience Department (MIND), Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"de la Torre","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Neuroscience Department (MIND), Scripps Research Institute, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00208-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.03.034"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2002.126297"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011500"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020051"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3429"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.10.025"},{"key":"e_1_3_2_9_2","first-page":"1792","volume":"2","year":"2007","unstructured":"Buchmeier, M. 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