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Certain tegument proteins can also regulate viral processes. For example, the pp71 tegument protein encoded by the UL82 gene of human cytomegalovirus (HCMV) stimulates viral immediate early (IE) gene expression and thus acts to initiate the productive lytic infectious cycle. In terminally differentiated fibroblasts infected with HCMV, tegument-delivered pp71 traffics to the nucleus and degrades the cellular transcriptional corepressor Daxx to initiate viral IE gene expression and lytic replication. However, when HCMV infects incompletely differentiated cells, tegument-delivered pp71 remains in the cytoplasm, allowing the nucleus-localized Daxx protein to silence viral IE gene expression and promote the establishment of a latent infection in certain cell types. We sought to determine whether undifferentiated cells block the trafficking of tegument-delivered pp71 to the nucleus or whether differentiated cells facilitate the nuclear transport of tegument-delivered pp71. Heterogenous cell fusion experiments demonstrated that tegument-delivered pp71 found in the cytoplasm of undifferentiated NT2 cells could be driven into the nucleus by one or more factors provided by fully differentiated fibroblasts. Our data raise the intriguing possibility that latency is the default program launched by HCMV upon viral entry into cells and that lytic infection is initiated only in certain (differentiated) cells that can facilitate the delivery of incoming pp71 to the nucleus.<\/jats:p>","DOI":"10.1128\/jvi.00500-10","type":"journal-article","created":{"date-parts":[[2010,8,5]],"date-time":"2010-08-05T03:53:47Z","timestamp":1280980427000},"page":"9853-9863","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Nuclear Localization of Tegument-Delivered pp71 in Human Cytomegalovirus-Infected Cells Is Facilitated by One or More Factors Present in Terminally Differentiated Fibroblasts"],"prefix":"10.1128","volume":"84","author":[{"given":"Rhiannon R.","family":"Penkert","sequence":"first","affiliation":[{"name":"Institute for Molecular Virology and McArdle Laboratory for Cancer Research, University of Wisconsin\u2014Madison, 1525 Linden Drive, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert F.","family":"Kalejta","sequence":"additional","affiliation":[{"name":"Institute for Molecular Virology and McArdle Laboratory for Cancer Research, University of Wisconsin\u2014Madison, 1525 Linden Drive, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0448"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.6.4599-4613.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.6.4400-4408.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2277"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.26.14506"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02676-05"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.12.7792-7802.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)84093-0"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.162299499"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00946-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C400479200"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1556\/AMicr.52.2005.3-4.11"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-66-3-509"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83019-0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.04.021"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00142-09"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-12-3087"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.11.5769-5783.2002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.3.1014"},{"key":"e_1_3_2_21_2","unstructured":"TMX2 blocks the replication of HSV-1 lacing ICP0 abstr. 1.06. 2009"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02639-08"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.05.037"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.1.5"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.2741\/jarvis"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.23.6682"},{"key":"e_1_3_2_27_2","first-page":"101","volume":"325","year":"2008","unstructured":"Kalejta, R. 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