{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:19:02Z","timestamp":1753881542604,"version":"3.41.2"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T00:00:00Z","timestamp":1502755200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI063928"],"award-info":[{"award-number":["AI063928"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001062","name":"Wallace H. Coulter Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2017,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Research on vaccine approaches that can provide long-term protection against dengue virus infection is needed. Here we describe the construction, immunogenicity, and preliminary information on the protective capacity of recombinant, replication-competent rhesus monkey rhadinovirus (RRV), a persisting herpesvirus. One RRV construct expressed nonstructural protein 5 (NS5), while a second recombinant expressed a soluble variant of the E protein (E85) of dengue virus 2 (DENV2). Four rhesus macaques received a single vaccination with a mixture of both recombinant RRVs and were subsequently challenged 19 weeks later with 1 \u00d7 10<jats:sup>5<\/jats:sup>PFU of DENV2. During the vaccine phase, plasma of all vaccinated monkeys showed neutralizing activity against DENV2. Cellular immune responses against NS5 were also elicited, as evidenced by major histocompatibility complex class I (MHC-I) tetramer staining in the one vaccinated monkey that was<jats:italic>Mamu-A*01<\/jats:italic>positive. Unlike two of two unvaccinated controls, two of the four vaccinated monkeys showed no detectable viral RNA sequences in plasma after challenge. One of these two monkeys also showed no anamnestic increases in antibody levels following challenge and thus appeared to be protected against the acquisition of DENV2 following high-dose challenge. Continued study will be needed to evaluate the performance of herpesviral and other persisting vectors for achieving long-term protection against dengue virus infection.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>Continuing studies of vaccine approaches against dengue virus (DENV) infection are warranted, particularly ones that may provide long-term immunity against all four serotypes. Here we investigated whether recombinant rhesus monkey rhadinovirus (RRV) could be used as a vaccine against DENV2 infection in rhesus monkeys. Upon vaccination, all animals generated antibodies capable of neutralizing DENV2. Two of four vaccinated monkeys showed no detectable viral RNA after subsequent high-dose DENV2 challenge at 19 weeks postvaccination. Furthermore, one of these vaccinated monkeys appeared to be protected against the acquisition of DENV2 infection on the basis of undetectable viral loads and the lack of an anamnestic antibody response. These findings underscore the potential utility of recombinant herpesviruses as vaccine vectors.<\/jats:p>","DOI":"10.1128\/jvi.00525-17","type":"journal-article","created":{"date-parts":[[2017,6,8]],"date-time":"2017-06-08T00:40:24Z","timestamp":1496882424000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Use of a Recombinant Gamma-2 Herpesvirus Vaccine Vector against Dengue Virus in Rhesus Monkeys"],"prefix":"10.1128","volume":"91","author":[{"given":"Georg F.","family":"Bischof","sequence":"first","affiliation":[{"name":"Department of Pathology, Miller School of Medicine, University of Miami, Miami, Florida, USA"},{"name":"Institute of Clinical and Molecular Virology, Friedrich Alexander Universit\u00e4t Erlangen-N\u00fcrnberg, Erlangen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1773-2406","authenticated-orcid":false,"given":"Diogo M.","family":"Magnani","sequence":"additional","affiliation":[{"name":"Department of Pathology, Miller School of Medicine, University of Miami, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Ricciardi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Miller School of Medicine, University of Miami, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young C.","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Pathology, Miller School of Medicine, University of Miami, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline","family":"Domingues","sequence":"additional","affiliation":[{"name":"Department of Pathology, Miller School of Medicine, University of Miami, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Varian K.","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Pathology, Miller School of Medicine, University of Miami, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Gonzalez-Nieto","sequence":"additional","affiliation":[{"name":"Department of Pathology, Miller School of Medicine, University of Miami, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva G.","family":"Rakasz","sequence":"additional","affiliation":[{"name":"Wisconsin National Primate Research Center, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David I.","family":"Watkins","sequence":"additional","affiliation":[{"name":"Department of Pathology, Miller School of Medicine, University of Miami, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald C.","family":"Desrosiers","sequence":"additional","affiliation":[{"name":"Department of Pathology, Miller School of Medicine, University of Miami, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001760"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/0470058005.ch2","article-title":"Dengue\/dengue haemorrhagic fever: history and current status","volume":"277","author":"Gubler DJ","year":"2006","unstructured":"Gubler DJ . 2006. 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