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In polyamine-depleted cells, bunyaviruses produce an overabundance of noninfectious particles that are indistinguishable from infectious particles. However, these particles interfere with productive infection and stimulate antiviral signaling pathways. We further find that additional enveloped viruses are similarly sensitive to polyamine depletion but that a nonenveloped enterovirus is not. We posit that polyamines are required to maintain bunyavirus infectivity and that polyamine depletion results in the accumulation of interfering noninfectious particles that limit infectivity. These results highlight a novel means by which bunyaviruses use polyamines for replication and suggest promising means to target host polyamines to reduce virus replication.<\/jats:p>","DOI":"10.1128\/jvi.00530-19","type":"journal-article","created":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T14:44:54Z","timestamp":1556808294000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Polyamine Depletion Inhibits Bunyavirus Infection via Generation of Noninfectious Interfering Virions"],"prefix":"10.1128","volume":"93","author":[{"given":"Vincent","family":"Mastrodomenico","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy J.","family":"Esin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and 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