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The objective of this study was to assess the interactions between MBL and severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein (SARS-S). MBL was found to selectively bind to retroviral particles pseudotyped with SARS-S. Unlike several other viral envelopes to which MBL can bind, both recombinant and plasma-derived human MBL directly inhibited SARS-S-mediated viral infection. Moreover, the interaction between MBL and SARS-S blocked viral binding to the C-type lectin, DC-SIGN. Mutagenesis indicated that a single N-linked glycosylation site, N330, was critical for the specific interactions between MBL and SARS-S. Despite the proximity of N330 to the receptor-binding motif of SARS-S, MBL did not affect interactions with the ACE2 receptor or cathepsin L-mediated activation of SARS-S-driven membrane fusion. Thus, binding of MBL to SARS-S may interfere with other early pre- or postreceptor-binding events necessary for efficient viral entry.<\/jats:p>","DOI":"10.1128\/jvi.00554-10","type":"journal-article","created":{"date-parts":[[2010,6,24]],"date-time":"2010-06-24T02:26:51Z","timestamp":1277346411000},"page":"8753-8764","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":137,"title":["A Single Asparagine-Linked Glycosylation Site of the Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Facilitates Inhibition by Mannose-Binding Lectin through Multiple Mechanisms"],"prefix":"10.1128","volume":"84","author":[{"given":"Yanchen","family":"Zhou","sequence":"first","affiliation":[{"name":"Blood Systems Research Institute and Department of Laboratory Medicine, University of California, San Francisco, San Francisco, California 94118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Lu","sequence":"additional","affiliation":[{"name":"Blood Systems Research Institute and Department of Laboratory Medicine, University of California, San Francisco, San Francisco, California 94118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Pfefferle","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Bertram","sequence":"additional","affiliation":[{"name":"Institute of Virology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilona","family":"Glowacka","sequence":"additional","affiliation":[{"name":"Institute of Virology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Drosten","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Bonn Medical Centre, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Po\u0308hlmann","sequence":"additional","affiliation":[{"name":"Institute of Virology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham","family":"Simmons","sequence":"additional","affiliation":[{"name":"Blood Systems Research Institute and Department of Laboratory Medicine, University of California, San Francisco, San Francisco, California 94118"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(90)90257-R"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.4049\/jimmunol.126.4.1562","volume":"126","year":"1981","unstructured":"Beebe, D. 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