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Pneumococci asymptomatically colonize the upper respiratory tract by forming biofilms. Viral infections, such as IAV, disrupt this stable colonization, leading to bacterial migration to the lower respiratory tract, and the development of severe pneumonia. However, the specific organs and tissues where IAV infection enhances the replication capacity of pneumococci in the biofilm state remain unclear. In this study, we engineered a mutant\n                      <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                      strain carrying the near-infrared luciferase gene,\n                      <jats:italic toggle=\"yes\">Akaluc<\/jats:italic>\n                      , which is selectively expressed during the log phase. Using this strain, we established a mouse model that enables\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      imaging of pneumococcal activation following IAV infection. In this model, IAV infection resulted in a marked increase in luminescence signals in the larynx, accompanied by increased bacterial proliferation in the lower respiratory tract. These results suggest that the larynx serves as a key site for pneumococcal activation and transition from biofilm to planktonic state during secondary bacterial pneumonia. Our imaging approach offers a powerful tool for elucidating host-pathogen interactions\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      and may contribute to the development of preventive strategies against secondary bacterial pneumonia following IAV infection.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Seasonal influenza virus infection increases the risk of severe bacterial pneumonia, but the underlying mechanisms remain unclear. In this study, we used a mouse model and a pneumococcal mutant strain expressing a near-infrared luminescent protein in the log phase, enabling\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        monitoring of the enhanced replication of pneumococci in deep tissues. We discovered that following influenza virus infection, bacterial replication is predominantly activated in the larynx, indicating that the larynx serves as a key site for activation of commensal pneumococci. This insight may inform the development of better strategies to prevent secondary bacterial pneumonia.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.00555-26","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:00:37Z","timestamp":1782738037000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Influenza A virus infection induces initial proliferation of commensal\n                    <i>Streptococcus pneumoniae<\/i>\n                    in the larynx leading to dissemination into the lower respiratory 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