{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T17:40:51Z","timestamp":1776447651338,"version":"3.51.2"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The uridylylation of the VPg peptide primer is the first stage in the replication of picornavirus RNA. This process can be achieved in vitro using purified components, including 3B (VPg) with the RNA dependent RNA polymerase (3D\n            <jats:sup>\n              <jats:italic>pol<\/jats:italic>\n            <\/jats:sup>\n            ), the precursor 3CD, and an RNA template containing the\n            <jats:italic>cre<\/jats:italic>\n            \/\n            <jats:italic>bus<\/jats:italic>\n            . We show that certain RNA sequences within the foot-and-mouth disease virus (FMDV) 5\u2032 untranslated region but outside of the\n            <jats:italic>cre\/bus<\/jats:italic>\n            can enhance VPg uridylylation activity. Furthermore, we have shown that the FMDV 3C protein alone can substitute for 3CD, albeit less efficiently. In addition, the VPg precursors, 3B\n            <jats:sub>3<\/jats:sub>\n            3C and 3B\n            <jats:sub>123<\/jats:sub>\n            3C, can function as substrates for uridylylation in the absence of added 3C or 3CD. Residues within the FMDV 3C protein involved in interaction with the\n            <jats:italic>cre\/bus<\/jats:italic>\n            RNA have been identified and are located on the face of the protein opposite from the catalytic site. These residues within 3C are also essential for VPg uridylylation activity and efficient virus replication.\n          <\/jats:p>","DOI":"10.1128\/jvi.00561-06","type":"journal-article","created":{"date-parts":[[2006,9,14]],"date-time":"2006-09-14T03:35:27Z","timestamp":1158204927000},"page":"9865-9875","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Role of RNA Structure and RNA Binding Activity of Foot-and-Mouth Disease Virus 3C Protein in VPg Uridylylation and Virus Replication"],"prefix":"10.1128","volume":"80","author":[{"given":"Arabinda","family":"Nayak","sequence":"first","affiliation":[{"name":"BBSRC Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian G.","family":"Goodfellow","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine, Imperial College, St Mary's Campus, London W2 1PG, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn E.","family":"Woolaway","sequence":"additional","affiliation":[{"name":"BBSRC Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Birtley","sequence":"additional","affiliation":[{"name":"Biophysics Section, Blackett Laboratory, Imperial College, South Kensington Campus, London SW7 2AZ, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Curry","sequence":"additional","affiliation":[{"name":"Biophysics Section, Blackett Laboratory, Imperial College, South Kensington Campus, London SW7 2AZ, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham J.","family":"Belsham","sequence":"additional","affiliation":[{"name":"BBSRC Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb06032.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.1.277-288.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.09.022"},{"key":"e_1_3_2_5_2","first-page":"869","year":"2000","unstructured":"Belsham, G. J., and R. J. Jackson. 2000. Translation initiation on picornavirus RNA, p. 869-900. In N. Sonenberg, J. W. B. Hershey, and M. B. Mathews (ed.), Translational control of gene expression. Monograph 39. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.","journal-title":"Translational control of gene expression"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.3.2436-2448.1997"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444905007924"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413254200"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0160"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.12.7803-7811.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.12.3132"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1583"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-8-1911"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.4.2251-2260.1992"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600971"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.22.10979-10990.2001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1999.1100"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.10.4590-4600.2000"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2950603"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19132-0"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.12.9490-9498.1997"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.34.3.627-634.1980"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11560"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.19.9686-9694.2002"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.3.1941-1952.1996"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838298981006"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.9.5136-5144.2003"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1306"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.8.4739-4750.2003"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.12.7698-7706.2005"},{"key":"e_1_3_2_31_2","first-page":"466","volume":"1","year":"1995","unstructured":"Pata, J. D., S. C. Shultz, and K. Kirkegaard. 1995. Functional oligomerization of poliovirus RNA dependent RNA polymerase. RNA1:466-477.","journal-title":"RNA"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204408200"},{"key":"e_1_3_2_33_2","first-page":"227","year":"2002","unstructured":"Paul, A. V. 2002. Possible unifying mechanism of picornavirus genome replication, p. 227-246. In B. L. Semler and E. Wimmer (ed.), Molecular biology of picornaviruses. ASM Press, Washington, D.C.","journal-title":"Molecular biology of picornaviruses"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.22.10359-10370.2000"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.2.891-904.2003"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307441200"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838299991240"},{"key":"e_1_3_2_38_2","unstructured":"Sambrook J. and D. W. Russell. 2001. Molecular cloning: a laboratory manual 3rd edn. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02256567"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.12.044"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403079101"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.3.2243-2246.2003"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.9.7191-7200.1998"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81297-0"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14510"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.8.6732-6741.1998"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.15.7485-7494.2002"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312992200"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.9.5152-5166.2003"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838202012785"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00561-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00561-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:28:55Z","timestamp":1646476135000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00561-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":49,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1128\/JVI.00561-06"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00561-06","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10]]},"assertion":[{"value":"2006-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-07-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}