{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:22:30Z","timestamp":1790533350330,"version":"4.1.0"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2018,8,29]],"date-time":"2018-08-29T00:00:00Z","timestamp":1535500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,9,15]],"date-time":"2018-09-15T00:00:00Z","timestamp":1536969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Association pour la recherche contre le cancer","award":["HFSPO RGP0044"],"award-info":[{"award-number":["HFSPO RGP0044"]}]},{"name":"Toulouse Tech Transfer","award":["Development project"],"award-info":[{"award-number":["Development project"]}]},{"DOI":"10.13039\/501100004097","name":"Fondation ARC","doi-asserted-by":"crossref","award":["ARC PJA"],"award-info":[{"award-number":["ARC PJA"]}],"id":[{"id":"10.13039\/501100004097","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2018,9,15]]},"abstract":"<jats:p>\n                    The ANCHOR technology is currently the most powerful tool to follow and quantify the replication of HCMV in living cells and to gain new insights into its biology. The technology is applicable to virtually any DNA virus or viruses presenting a double-stranded DNA (dsDNA) phase, paving the way to imaging infection in various cell lines, or even in animal models, and opening fascinating fundamental and applied prospects. Associated with high-content automated microscopy, the technology permitted rapid, robust, and precise determination of ganciclovir 50% and 90% inhibitory concentrations (IC\n                    <jats:sub>50<\/jats:sub>\n                    and IC\n                    <jats:sub>90<\/jats:sub>\n                    ) on HCMV replication, with minimal hands-on time investment. To search for new antiviral activities, the experiment is easy to upgrade toward efficient and cost-effective screening of large chemical libraries. Simple infection of permissive cells with ANCHOR viruses in the presence of a compound of interest even provides a first estimation of the stage of the viral cycle the molecule is acting upon.\n                  <\/jats:p>","DOI":"10.1128\/jvi.00571-18","type":"journal-article","created":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T06:19:16Z","timestamp":1529389156000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Real-Time Visualization and Quantification of Human Cytomegalovirus Replication in Living Cells Using the ANCHOR DNA Labeling Technology"],"prefix":"10.1128","volume":"92","author":[{"given":"Bernard","family":"Mariam\u00e9","sequence":"first","affiliation":[{"name":"Laboratoire de Biologie Mol\u00e9culaire Eucaryote (LBME), Centre de Biologie Int\u00e9grative (CBI), University of Toulouse, CNRS, UPS, Toulouse, France"},{"name":"Institute for Advanced Life Science Technology (ITAV), University of Toulouse, CNRS, UPS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Kappler-Gratias","sequence":"additional","affiliation":[{"name":"Institute for Advanced Life Science Technology (ITAV), University of Toulouse, CNRS, UPS, Toulouse, France"},{"name":"NeoVirTech SAS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kappler","sequence":"additional","affiliation":[{"name":"statalpha, Bazi\u00e8ge, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Balor","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Mol\u00e9culaire Eucaryote (LBME), Centre de Biologie Int\u00e9grative (CBI), University of Toulouse, CNRS, UPS, Toulouse, France"},{"name":"Multiscale Electron Imaging (METi) Facility, Centre de Biologie Integrative (CBI), University of Toulouse, CNRS, UPS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Gallardo","sequence":"additional","affiliation":[{"name":"Institute for Advanced Life Science Technology (ITAV), University of Toulouse, CNRS, UPS, Toulouse, France"},{"name":"NeoVirTech SAS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Bystricky","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Mol\u00e9culaire Eucaryote (LBME), Centre de Biologie Int\u00e9grative (CBI), University of Toulouse, CNRS, UPS, Toulouse, France"},{"name":"Institute for Advanced Life Science Technology (ITAV), University of Toulouse, CNRS, UPS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"2703","volume-title":"Fields virology","author":"Mocarski EJ","year":"2007","unstructured":"Mocarski EJ, Shenk T, Pass RF. 2007. Cytomegaloviruses, p 2703\u20132774. In Knipe DM, Howley PM (ed), Fields virology, 5th ed. Lippincott, Williams, & Wilkins, Philadelphia, PA.","edition":"5"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181644b70"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.655"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.535"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.3.1761-1764.1992"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.11.6576-6585.1993"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.12.9803-9807.1997"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00447-8"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01818"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.82"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-76-4-741"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-12-3099"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top065623"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2013.01.005"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2011.02.006"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061029798"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.20.11082-11093.2003"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.5.2754-2767.2005"},{"key":"e_1_3_3_20_2","unstructured":"Bystricky K Gallardo F Lane D Dubarry N. 2012. Constructs and method for regulating gene expression or for detecting and controlling a DNA locus in eukaryotes. Patent WO\/2012\/127047. https:\/\/patentscope.wipo.int\/search\/en\/detail.jsf?docId=WO2012127047."},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.242206.114"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2015.07.013"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004187"},{"key":"e_1_3_3_24_2","doi-asserted-by":"crossref","unstructured":"Germier T Kocanova S Bancaud A Walthers N Sellou H Shaban H Ellenberg J Gallardo F Bystricky K. 2017. Real-time chromatin dynamics of the single gene level during transcription activation. bioRxiv http:\/\/biorxiv.org\/content\/early\/2017\/02\/23\/111179.","DOI":"10.1101\/111179"},{"key":"e_1_3_3_25_2","doi-asserted-by":"crossref","unstructured":"Mariam\u00e9 B Kappler-Gratias S Kappler M Balor S Gallardo F Bystricky K. 2018. Real-time visualization and quantification of human cytomegalovirus replication in living cells using the ANCHOR DNA labeling technology. bioRxiv. doi:10.1101\/300111.","DOI":"10.1101\/300111"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001256"},{"key":"e_1_3_3_27_2","first-page":"273","article-title":"Human cytomegalovirus infection of cultured fibroblasts. II. Viral replicative sequence of a wild and an adapted strain","volume":"27","author":"Kanich RE","year":"1972","unstructured":"Kanich RE, Craighead JE. 1972. Human cytomegalovirus infection of cultured fibroblasts. II. Viral replicative sequence of a wild and an adapted strain. Lab Invest 27:273\u2013282.","journal-title":"Lab Invest"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-181-42274"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8848"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.2.975-986.2000"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.8.3842-3851.2000"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01077-07"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2008.02059.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.23.14660-14667.2005"},{"key":"e_1_3_3_35_2","volume-title":"Human herpesviruses: biology, therapy, and immunoprophylaxis","author":"Britt B","year":"2007","unstructured":"Britt B. 2007. Maturation and egress. Chapter 20. In Arvin A, Campadelli-Fiume G, Mocarski E, Moore PS, Roizman B, Whitley R, Yamanishi K (ed), Human herpesviruses: biology, therapy, and immunoprophylaxis. Cambridge University Press, Cambridge, United Kingdom."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3390\/v7030899"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.09.020"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(00)00273-1"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1987.03390220080025"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.3.488"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1086\/516445"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/168.2.332"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02858-15"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1998.4055"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3344"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam6892"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)01436-7"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.01.020"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90141-9"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.3.1751-1758.1996"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.2.1040-1051.1994"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.1987.Supplement_7.6"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000063"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83286-0"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.10.8320-8329.1999"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc14e03s18"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00934-6"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-98141-3"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471143030.cb0408s11"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-801075-4.00018-5"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00795-18"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00571-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.00571-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:38:42Z","timestamp":1646473122000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.00571-18"}},"subtitle":[],"editor":[{"given":"Jae U.","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,9,15]]},"references-count":60,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2018,9,15]]}},"alternative-id":["10.1128\/JVI.00571-18"],"URL":"https:\/\/doi.org\/10.1128\/jvi.00571-18","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/300111","asserted-by":"object"}]},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,15]]},"assertion":[{"value":"2018-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00571-18"}}